Davilla elliptica and Davilla nitida: Gastroprotective, anti-inflammatory immunomodulatory and anti- Helicobacter pylori action

• Published in: Journal of ethnopharmacology 2009-06, Vol.123 (3), p.430-438
• Main Authors: Kushima, Hélio, Nishijima, Catarine Massucato, Rodrigues, Clenilson Martins, Rinaldo, Daniel, Sassá, Micheli Fernanda, Bauab, Taís Maria, Stasi, Luiz Claudio Di, Carlos, Iracilda Zeppone, Brito, Alba Regina Monteiro Souza, Vilegas, Wagner, Hiruma-Lima, Clélia Akiko
• Format: Article
• Language: English
• Subjects: Animals; Anti- Helicobacter pylori activity; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Anti-Inflammatory Agents, Non-Steroidal; Anti-Ulcer Agents - pharmacology; Anti-Ulcer Agents - therapeutic use; Biological and medical sciences; Davilla elliptica St. Hil; Davilla nitida (Vahl) Kubitzki; Dilleniaceae; Disease Models, Animal; Edema - drug therapy; Ethanol; Gastric Mucosa - drug effects; Gastric protection; General pharmacology; Glutathione; Glutathione - metabolism; Helicobacter Infections - drug therapy; Helicobacter pylori; Helicobacter pylori - drug effects; Hydrochloric Acid; Immunologic Factors - pharmacology; Immunologic Factors - therapeutic use; Macrophages - drug effects; Male; Medical sciences; Mice; Nitric Oxide - metabolism; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Rats; Rats, Wistar; Stomach Ulcer - chemically induced; Stomach Ulcer - drug therapy; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2009.03.031

Davilla elliptica and Davilla nitida are species commonly found in the Brazilian Cerrado biome. Based on ethnopharmacological and phytochemical analyses, methanolic extracts from leaves of Davilla elliptica (EDE) and Davilla nitida (EDN) were evaluated for their anti-ulcer, anti-inflammatory, immunological and anti- Helicobacter pylori activities. The gastroprotective action of both extracts was evaluated in rodent experimental models (HCl/ethanol, ethanol or NSAID). We also evaluated anti-inflammatory (carrageenin-induced rat hind paw edema), immunomodulatory (murine peritoneal macrophages) and antibacterial action of both extracts against a standard strain of Helicobacter pylori. EDE and EDN (500 mg/kg) were able to protect gastric mucosa against HCl/ethanol solution (EDE 63%; EDN 59%), absolute ethanol (EDE 95%; EDN 88%), and also against injurious effect of NSAID (EDE 77%; EDN 67%). When EDE and EDN were challenged with sulfhydryl depleter compound, the gastroprotective action of both extracts was completely abolished. EDE had gastroprotective effect related to increase of glutathione bioavailability and stimulated higher levels of NO, H 2O 2 and TNF-α production. Otherwise EDN showed better anti- Helicobacter pylori action than EDE. Neither extracts presented anti-inflammatory activity by oral route. The phytochemical investigation showed that both extracts possess phenolic acid derivatives, acylglycoflavonoids and condensed tannins with evident quantitative variations that probably influenced the pharmacological differences between extracts.