Genetic mapping and identification of genes for important traits using a genomic BAC library

The genetic control of economically important traits in spinach (Spinacia oleraceae) is not well understood. In an effort to develop a better understanding of the genetic control of important traits in spinach and to advance the potential for marker assisted selection in breeding programs, a genomic...

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Bibliographic Details
Published in:Phytopathology 2006-06, Vol.96 (6), p.S115-S115
Main Authors: Tomlinson, AN, Correll, J C, Feng, C, Irish, B M
Format: Article
Language:English
Subjects:
Albugo
Peronospora
Spinacia
Spinacia oleracea
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Summary:The genetic control of economically important traits in spinach (Spinacia oleraceae) is not well understood. In an effort to develop a better understanding of the genetic control of important traits in spinach and to advance the potential for marker assisted selection in breeding programs, a genomic bacteria artificial chromosome (BAC) library is being developed. Approximately 33,000 BIBAC clones, with an average insert size of 150 kb, will be needed for a 5X coverage of the spinach genome, which is composed of 6 chromosomes and 989 Mb. To further facilitate examination of the spinach genome, genetic mapping populations are being developed by crossing various parental spinach genotypes either resistant or susceptible to the downy mildew (Peronospora farinosa f. sp. spinaciae) and white rust (Albugo occidentalis) pathogens. Previously, a marker (Dm-1) was identified that was tightly linked to the Pfs-1 resistance locus for race 6 of the downy mildew pathogen in spinach. The spinach BAC library is being used to identify the resistance gene residing at the Pfs-1 locus. Additional molecular markers are being developed from spinach and related species to construct the genetic map.
ISSN:0031-949X