A validated method for analysis of chromium picolinate in nutraceuticals by reversed phase high performance liquid chromatography

A validated high performance liquid chromatographic method was developed for the determination of chromium picolinate in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed‐phase Supelcosil LC‐18 (250 × 4.6 mm, 5 µm) column. The mobile phase consisted of acet...

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Bibliographic Details
Published in:Biomedical chromatography 2005-03, Vol.19 (2), p.119-122
Main Authors: Koll, Michael, Hoenen, Hubertus, Aboul-Enein, Hassan Y.
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
chromium picolinate
dietary supplement
Dietary Supplements - analysis
Drug Stability
high-performance liquid chromatography
Iron Chelating Agents - analysis
nutraceutical analysis
Picolinic Acids - analysis
Picolinic Acids - toxicity
Regression Analysis
Sensitivity and Specificity
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Summary:A validated high performance liquid chromatographic method was developed for the determination of chromium picolinate in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed‐phase Supelcosil LC‐18 (250 × 4.6 mm, 5 µm) column. The mobile phase consisted of acetonitrile:water (40:60 v/v) at a flow rate of 0.8 mL/min. The UV‐detector was set at 264 nm. The developed method showed a good linear relationship in the concentration range from 0.125 to 12.5 µg/mL with a correlation coefficient from 0.999. The limit of detection and limit of quantification were 0.091 and 0.181 µg/mL, respectively. Copyright © 2004 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.424