Host serum microRNA profiling during the early stage of foot-and-mouth disease virus infection

Foot-and-mouth disease virus (FMDV) causes a highly contagious infection in cloven-hoofed animals, with many outbreaks in the developing world. MicroRNAs (miRNAs) are non-coding RNAs that regulate antiviral defence by post-transcriptional regulation of gene expression. In this study, the host miRNA...

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Bibliographic Details
Published in:Archives of virology 2018-08, Vol.163 (8), p.2055-2063
Main Authors: Basagoudanavar, Suresh H., Hosamani, Madhusudan, Tamil Selvan, R. P., Sreenivasa, B. P., Sanyal, Aniket, Venkataramanan, R.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cell culture
Foot & mouth disease
Gene expression
Gene regulation
Genomes
Homeostasis
Immune response
Infections
Infectious Diseases
Medical Microbiology
miRNA
Nucleotide sequence
Original Article
Outbreaks
Post-transcription
Signal transduction
Virology
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Summary:Foot-and-mouth disease virus (FMDV) causes a highly contagious infection in cloven-hoofed animals, with many outbreaks in the developing world. MicroRNAs (miRNAs) are non-coding RNAs that regulate antiviral defence by post-transcriptional regulation of gene expression. In this study, the host miRNA response following FMDV infection was investigated in cattle, a natural host for FMDV. A significant alteration in serum miRNA expression was detected at early stages of infection. Compared to prior to infection, on day 2 postinfection (PI), 119 miRNAs were upregulated, of which 39 were significantly upregulated ( P < 0.05). Gene target prediction and pathway enrichment analysis suggested that upregulated miRNAs target innate immune signalling pathways, suggesting a homeostasis effect, possibly to limit inappropriate immune responses. Further, for the significantly upregulated miRNAs, nine miRNA recognition elements were identified in the genome sequence of FMDV serotype O, which was used for infection. The antiviral effect of four of these miRNAs was confirmed in a cell culture system. These data demonstrate that changes in miRNA expression occur during early pathogenesis, and the identification of possible miRNA targets genes could help in elucidating molecular events involved in virus-host interaction and thus could be useful in developing therapeutic strategies.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-018-3824-8