Comparison of Various Measures of Microbial Growth Kinetics in Suspended and Biofilm Cultures during Biodegradation of Naphthalene

This research focused on measurement of bacterial growth rates and activities in suspended cultures and biofilms using Pseudomonas putida (ATCC 17484) for biodegradation of naphthalene. A comparison was made of the effect of three biomass measures: optical density, total cell protein, and living cel...

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Bibliographic Details
Published in:Water environment research 2002-05, Vol.74 (3), p.272-279
Main Authors: Shim, Jeong Seop, Lewandowski, Gordon A.
Format: Article
Language:English
Subjects:
Applied sciences
Bacteria
Biodegradation
Biodegradation, Environmental
BIOFILM
Biofilms
Biological and medical sciences
Biological treatment of waters
Biomass
BIOMASS MEASURES
Biomass production
Biotechnology
Cell membranes
CELL VIABILITY
CONTINUOUS FLOW
E coli
Environment and pollution
EPIFLUORESCENCE MICROSCOPY
Exact sciences and technology
Flasks
Fluorescence
FLUORESCENT STAINING
Fundamental and applied biological sciences. Psychology
General purification processes
Industrial applications and implications. Economical aspects
Kinetics
Methods
MICROBIAL GROWTH KINETICS
Microscopy
NAPHTHALENE
Naphthalenes - metabolism
Optical density
Pollution
Population Dynamics
Proteins
Pseudomonas putida
PSEUDOMONAS PUTIDA (ATCC 17484)
Pseudomonas putida - physiology
Research Note
SUSPENDED CULTURE
Viability
Wastewaters
Water Purification - methods
Water treatment and pollution
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Summary:This research focused on measurement of bacterial growth rates and activities in suspended cultures and biofilms using Pseudomonas putida (ATCC 17484) for biodegradation of naphthalene. A comparison was made of the effect of three biomass measures: optical density, total cell protein, and living cell number on the calculated rate of naphthalene disappearance. Living cell number was determined by optimizing a fluorescent staining technique and using epifluorescence microscopy. All three techniques gave similar results in terms of the specific growth rates, and in all three there was a lag time between the disapperance of naphthalene and the appearance of biomass. This was most likely a consequence of the production of intermediate products detected in the chromatograms. Inclusion of a one-hour lag time in the integrated Monod expression considerably improved the agreement between experimental and calculated values. However, further improvements to an understanding of the overall rate processes may require more detailed kinetics of the individual biochemical pathways.
ISSN:1061-4303
1554-7531
DOI:10.2175/106143002X140008