Aglycosylated immunoglobulin G sub(1) variants productively engage activating Fc receptors

• Published in: Proceedings of the National Academy of Sciences - PNAS 2008-12, Vol.105 (51), p.20167-20172
• Main Authors: Sazinsky, Stephen L, Ott, Rene G, Silver, Nathaniel W, Tidor, Bruce, Ravetch, Jeffrey V, Wittrup, KDane
• Format: Article
• Language: English
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0809257105

Immunoglobulin G plays a vital role in adaptive immunity and antibody-based therapy through engagement of its Fc region by the Fc gamma receptors (Fc gamma Rs) on immune cells. In addition to specific protein-protein contacts, N-linked glycosylation of the IgG Fc has been thought to be essential for the recognition of Fc by Fc gamma R. This requirement for the N- linked glycan has limited biomanufacture of therapeutic antibodies by restricting it to mammalian expression systems. We report here aglycosylated Fc domain variants that maintain engagement to Fc gamma Rs, both in vitro and in vivo, demonstrating that Fc glycosylation is not strictly required for the activation of immune cells by IgG. These variants provide insight into how the N-linked glycan is used biologically in the recognition of Fc by Fc gamma Rs, as well as represent a step toward the production in alternative expression systems of antibody-based therapeutics capable of eliciting immune effector functions.