LncRNA PlncRNA-1 overexpression inhibits the growth of breast cancer by upregulating TGF-β1 and downregulating PHGDH

• Published in: Breast cancer (Tokyo, Japan) Japan), 2018-09, Vol.25 (5), p.619-625
• Main Authors: Li, Qing, Gao, Haifeng, Zhou, Shuyan, Liao, Yuting
• Format: Article
• Language: English
• Subjects: Cancer Research; Medicine; Medicine & Public Health; Oncology; Rapid Communication; Surgery; Surgical Oncology
• ISSN: 1340-6868; 1880-4233
• DOI: 10.1007/s12282-018-0858-4

Objective To investigate the role of lncRNA PlncRNA-1 in the pathogenesis of breast cancer. Methods A total of 78 patients with breast cancer as well as 48 healthy females were included in this study. Expression in tumor tissues and adjacent healthy tissues of breast cancer patients, as well as in breast tissues and serum of both patients and healthy control was detected by qRT-PCR. Cell proliferation was detected by CCK-8 assay, and cell apoptosis was tested by MTT assay. PlncRNA-1 overexpression cell lines were constructed and the effects on TGF-β1 as well as phosphoglycerate dehydrogenase (PHGDH) were explored by western blot. Results Expression levels of PlncRNA-1 were significantly lower in tumor tissues than those in adjacent healthy tissues. Significantly lower expression levels of PlncRNA-1 were also found in breast cancer patients than those in healthy controls in both breast tissue and serum. Upregulation of PlncRNA-1 promoted the expression of TGF-β1, but inhibited the expression of PHGDH. LncRNA PlncRNA-1 overexpression reduced the proliferation rate, but increased the apoptosis rate of breast cancer cells, while treatment with TGF-β inhibitor reduced those effects of PlncRNA-1 overexpression. Conclusion LncRNA PlncRNA-1 overexpression inhibits the growth of breast cancer by upregulating TGF-β1 and downregulating PHGDH.