Chromatin Accessibility Dynamics during Chemical Induction of Pluripotency

• Published in: Cell stem cell 2018-04, Vol.22 (4), p.529-542.e5
• Main Authors: Cao, Shangtao, Yu, Shengyong, Li, Dongwei, Ye, Jing, Yang, Xuejie, Li, Chen, Wang, Xiaoshan, Mai, Yuanbang, Qin, Yue, Wu, Jian, He, Jiangping, Zhou, Chunhua, Liu, He, Zhao, Bentian, Shu, Xiaodong, Wu, Chuman, Chen, Ruiping, Chan, Waiyee, Pan, Guangjin, Chen, Jiekai, Liu, Jing, Pei, Duanqing
• Format: Article
• Language: English
• Subjects: AP1; bromodeoxyuridine; cell fate decision; chromatin accessibility; reprogramming; small molecules
• ISSN: 1934-5909; 1875-9777
• DOI: 10.1016/j.stem.2018.03.005

Despite its exciting potential, chemical induction of pluripotency (CIP) efficiency remains low and the mechanisms are poorly understood. We report the development of an efficient two-step serum- and replating-free CIP protocol and the associated chromatin accessibility dynamics (CAD) by assay for transposase-accessible chromatin (ATAC)-seq. CIP reorganizes the somatic genome to an intermediate state that is resolved under 2iL condition by re-closing previously opened loci prior to pluripotency acquisition with gradual opening of loci enriched with motifs for the OCT/SOX/KLF families. Bromodeoxyuridine, a critical ingredient of CIP, is responsible for both closing and opening critical loci, at least in part by preventing the opening of loci enriched with motifs for the AP1 family and facilitating the opening of loci enriched with SOX/KLF/GATA motifs. These changes differ markedly from CAD observed during Yamanaka-factor-driven reprogramming. Our study provides insights into small-molecule-based reprogramming mechanisms and reorganization of nuclear architecture associated with cell-fate decisions. [Display omitted] •CIP occurs in two stages mediated by serum-free chemical cocktail and 2iL•ATAC-seq-based chromatin accessibility dynamics in CIP differ from OSK reprogramming•CAD highlight the role of GATAs, SOXs, and FOXs during CIP•CADs with or without BrdU reveal a key role in reorganizing chromatin Cao et al. report an efficient protocol for chemical induction of pluripotency and its chromatin accessibility dynamics, which links small molecules and the reorganization of nuclear architecture in the context of cell-fate decisions.