Loading…

In vitro photodynamic inactivation effects of benzylidene cyclopentanone photosensitizers on clinical fluconazole-resistant Candida albicans

•The three BCB PSs all have remarkable PDI effects on C. albicans.•BCB PSs-PDI was not affected by the fluconazole susceptibility of C. albicans.•The respective subcellular localization of PSs led to different PDI mechanisms.•The best effect is obtained by P1, which is lipophilic and has one cationi...

Full description

Saved in:
Bibliographic Details
Published in:Photodiagnosis and photodynamic therapy 2018-06, Vol.22, p.178-186
Main Authors: Zhou, Shaona, Sun, Zhiyuan, Ye, Zulin, Wang, Ying, Wang, Leili, Xing, Limei, Qiu, Haixia, Huang, Naiyan, Luo, Yanping, Zhao, Yuxia, Gu, Ying
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•The three BCB PSs all have remarkable PDI effects on C. albicans.•BCB PSs-PDI was not affected by the fluconazole susceptibility of C. albicans.•The respective subcellular localization of PSs led to different PDI mechanisms.•The best effect is obtained by P1, which is lipophilic and has one cationic charge. The incidence of Candida infections has increased for various reasons, including, the more frequent use of immunosuppresants or broad-spectrum antibiotics. Photodynamic inactivation (PDI) is a promising approach for treating localized Candida infections. The PDI efficacies of three benzylidene cyclopentanone-based (BCB) photosensitizers (PSs: P1, P2 and Y1) against three fluconazole-resistant C. albicans (cal-1, cal-2, and cal-3) and one control C. albicans (ATCC 90028), respectively, were evaluated using an established plate dilution method. The binding of PSs to C. albicans was determined by fluorescence spectroscopy. The mechanism of antifungal PDI was investigated using confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). Three BCB PSs all bound rapidly to C. albicans. After incubation with PSs for 30 min and irradiation with a 532 nm laser for 10 min (40 mW cm−2, 24 J cm−2), the fungicidal activity was achieved as 7.5 μM for P1 and P2, and 25 μM for Y1. CLSM confirmed that P1 and Y1 were located in intracellular components, including mitochondria, while P2 bound to the protoplast exterior and failed to enter the cells. TEM revealed the damage of mitochondria ultrastructures after P1- or Y1-mediated PDI, consistenting with the CLSM results. However, most cells became edematous, enlarged or deformation after P2-mediated PDI. The three BCB PSs all have remarkable PDI effects on C. albicans. The best effect is obtained by P1, which has one cationic charge with a proper lipophilicity. The respective subcellular localization of the three PSs led to different PDI mechanisms.
ISSN:1572-1000
1873-1597
DOI:10.1016/j.pdpdt.2018.04.001