Evaluation of the intracellular activity of drugs against Mycobacterium abscessus using a THP-1 macrophage model

We evaluated the antimicrobial effectiveness against M. abscessus in a THP-1 cell line model. No intracellular activity was observed when using amikacin or imipenem. A bacteriostatic effect was observed for cefoxitin, clarithromycin and azithromycin. Tigecycline showed the best antibacterial effect...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2018-05, Vol.148, p.29-32
Main Authors: Molina-Torres, Carmen Amelia, Tamez-Peña, Lorena, Castro-Garza, Jorge, Ocampo-Candiani, Jorge, Vera-Cabrera, Lucio
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - pharmacokinetics
Anti-Bacterial Agents - pharmacology
Humans
Intracellular activity
Macrophages - metabolism
Macrophages - microbiology
Microbial Viability - drug effects
Models, Biological
Mycobacterium abscessus
Mycobacterium abscessus - drug effects
Mycobacterium abscessus - growth & development
Mycobacterium Infections, Nontuberculous - drug therapy
THP-1 Cells
THP-1 macrophages
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c359t-34dfb7ee186f0b80532ab2b15ec1d852433ed44c5726444d3d12383242b552f43
cites cdi_FETCH-LOGICAL-c359t-34dfb7ee186f0b80532ab2b15ec1d852433ed44c5726444d3d12383242b552f43
container_end_page 32
container_issue
container_start_page 29
container_title Journal of microbiological methods
container_volume 148
creator Molina-Torres, Carmen Amelia
Tamez-Peña, Lorena
Castro-Garza, Jorge
Ocampo-Candiani, Jorge
Vera-Cabrera, Lucio
description We evaluated the antimicrobial effectiveness against M. abscessus in a THP-1 cell line model. No intracellular activity was observed when using amikacin or imipenem. A bacteriostatic effect was observed for cefoxitin, clarithromycin and azithromycin. Tigecycline showed the best antibacterial effect by decreasing the intracellular growth up to bactericidal level.
doi_str_mv 10.1016/j.mimet.2018.03.020
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2022983293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0167701218301623</els_id><sourcerecordid>2022983293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-34dfb7ee186f0b80532ab2b15ec1d852433ed44c5726444d3d12383242b552f43</originalsourceid><addsrcrecordid>eNp9kDtvFDEURi0EIpvAL0CKXNLMxL62Z2YLiigKJFIQFKG2PPadjVfz2Pix0v57vGxImeoW93z3cQj5wlnNGW-utvXkJ0w1MN7VTNQM2Duy4l0LVSfU-j1ZFaqtWsbhjJzHuGWMKyG7j-QM1g00qmlXZHe7N2M2yS8zXQaanpD6OQVjcRzzaAI1Nvm9T4dj14W8idRsjJ9joj8PdulLG4PPEzV9tBhjjjRHP2-ooY93vytOJ2PDsnsyG6TT4nD8RD4MZoz4-aVekD_fbx9v7qqHXz_ub64fKltuT5WQbuhbRN41A-s7pgSYHnqu0HLXKZBCoJPSqhYaKaUTjoPoBEjolYJBigvy9TR3F5bnjDHpycfjV2bGJUcNDGBdAmtRUHFCy6UxBhz0LvjJhIPmTB9V663-p1ofVWsmdFFdUpcvC3I_oXvN_HdbgG8nAMube49BR-txtuh8QJu0W_ybC_4CUx6RLw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2022983293</pqid></control><display><type>article</type><title>Evaluation of the intracellular activity of drugs against Mycobacterium abscessus using a THP-1 macrophage model</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Molina-Torres, Carmen Amelia ; Tamez-Peña, Lorena ; Castro-Garza, Jorge ; Ocampo-Candiani, Jorge ; Vera-Cabrera, Lucio</creator><creatorcontrib>Molina-Torres, Carmen Amelia ; Tamez-Peña, Lorena ; Castro-Garza, Jorge ; Ocampo-Candiani, Jorge ; Vera-Cabrera, Lucio</creatorcontrib><description>We evaluated the antimicrobial effectiveness against M. abscessus in a THP-1 cell line model. No intracellular activity was observed when using amikacin or imipenem. A bacteriostatic effect was observed for cefoxitin, clarithromycin and azithromycin. Tigecycline showed the best antibacterial effect by decreasing the intracellular growth up to bactericidal level.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2018.03.020</identifier><identifier>PMID: 29626567</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - administration &amp; dosage ; Anti-Bacterial Agents - pharmacokinetics ; Anti-Bacterial Agents - pharmacology ; Humans ; Intracellular activity ; Macrophages - metabolism ; Macrophages - microbiology ; Microbial Viability - drug effects ; Models, Biological ; Mycobacterium abscessus ; Mycobacterium abscessus - drug effects ; Mycobacterium abscessus - growth &amp; development ; Mycobacterium Infections, Nontuberculous - drug therapy ; THP-1 Cells ; THP-1 macrophages</subject><ispartof>Journal of microbiological methods, 2018-05, Vol.148, p.29-32</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-34dfb7ee186f0b80532ab2b15ec1d852433ed44c5726444d3d12383242b552f43</citedby><cites>FETCH-LOGICAL-c359t-34dfb7ee186f0b80532ab2b15ec1d852433ed44c5726444d3d12383242b552f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29626567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Molina-Torres, Carmen Amelia</creatorcontrib><creatorcontrib>Tamez-Peña, Lorena</creatorcontrib><creatorcontrib>Castro-Garza, Jorge</creatorcontrib><creatorcontrib>Ocampo-Candiani, Jorge</creatorcontrib><creatorcontrib>Vera-Cabrera, Lucio</creatorcontrib><title>Evaluation of the intracellular activity of drugs against Mycobacterium abscessus using a THP-1 macrophage model</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>We evaluated the antimicrobial effectiveness against M. abscessus in a THP-1 cell line model. No intracellular activity was observed when using amikacin or imipenem. A bacteriostatic effect was observed for cefoxitin, clarithromycin and azithromycin. Tigecycline showed the best antibacterial effect by decreasing the intracellular growth up to bactericidal level.</description><subject>Anti-Bacterial Agents - administration &amp; dosage</subject><subject>Anti-Bacterial Agents - pharmacokinetics</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Humans</subject><subject>Intracellular activity</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - microbiology</subject><subject>Microbial Viability - drug effects</subject><subject>Models, Biological</subject><subject>Mycobacterium abscessus</subject><subject>Mycobacterium abscessus - drug effects</subject><subject>Mycobacterium abscessus - growth &amp; development</subject><subject>Mycobacterium Infections, Nontuberculous - drug therapy</subject><subject>THP-1 Cells</subject><subject>THP-1 macrophages</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kDtvFDEURi0EIpvAL0CKXNLMxL62Z2YLiigKJFIQFKG2PPadjVfz2Pix0v57vGxImeoW93z3cQj5wlnNGW-utvXkJ0w1MN7VTNQM2Duy4l0LVSfU-j1ZFaqtWsbhjJzHuGWMKyG7j-QM1g00qmlXZHe7N2M2yS8zXQaanpD6OQVjcRzzaAI1Nvm9T4dj14W8idRsjJ9joj8PdulLG4PPEzV9tBhjjjRHP2-ooY93vytOJ2PDsnsyG6TT4nD8RD4MZoz4-aVekD_fbx9v7qqHXz_ub64fKltuT5WQbuhbRN41A-s7pgSYHnqu0HLXKZBCoJPSqhYaKaUTjoPoBEjolYJBigvy9TR3F5bnjDHpycfjV2bGJUcNDGBdAmtRUHFCy6UxBhz0LvjJhIPmTB9V663-p1ofVWsmdFFdUpcvC3I_oXvN_HdbgG8nAMube49BR-txtuh8QJu0W_ybC_4CUx6RLw</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Molina-Torres, Carmen Amelia</creator><creator>Tamez-Peña, Lorena</creator><creator>Castro-Garza, Jorge</creator><creator>Ocampo-Candiani, Jorge</creator><creator>Vera-Cabrera, Lucio</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201805</creationdate><title>Evaluation of the intracellular activity of drugs against Mycobacterium abscessus using a THP-1 macrophage model</title><author>Molina-Torres, Carmen Amelia ; Tamez-Peña, Lorena ; Castro-Garza, Jorge ; Ocampo-Candiani, Jorge ; Vera-Cabrera, Lucio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-34dfb7ee186f0b80532ab2b15ec1d852433ed44c5726444d3d12383242b552f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anti-Bacterial Agents - administration &amp; dosage</topic><topic>Anti-Bacterial Agents - pharmacokinetics</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Humans</topic><topic>Intracellular activity</topic><topic>Macrophages - metabolism</topic><topic>Macrophages - microbiology</topic><topic>Microbial Viability - drug effects</topic><topic>Models, Biological</topic><topic>Mycobacterium abscessus</topic><topic>Mycobacterium abscessus - drug effects</topic><topic>Mycobacterium abscessus - growth &amp; development</topic><topic>Mycobacterium Infections, Nontuberculous - drug therapy</topic><topic>THP-1 Cells</topic><topic>THP-1 macrophages</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Molina-Torres, Carmen Amelia</creatorcontrib><creatorcontrib>Tamez-Peña, Lorena</creatorcontrib><creatorcontrib>Castro-Garza, Jorge</creatorcontrib><creatorcontrib>Ocampo-Candiani, Jorge</creatorcontrib><creatorcontrib>Vera-Cabrera, Lucio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Molina-Torres, Carmen Amelia</au><au>Tamez-Peña, Lorena</au><au>Castro-Garza, Jorge</au><au>Ocampo-Candiani, Jorge</au><au>Vera-Cabrera, Lucio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the intracellular activity of drugs against Mycobacterium abscessus using a THP-1 macrophage model</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2018-05</date><risdate>2018</risdate><volume>148</volume><spage>29</spage><epage>32</epage><pages>29-32</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>We evaluated the antimicrobial effectiveness against M. abscessus in a THP-1 cell line model. No intracellular activity was observed when using amikacin or imipenem. A bacteriostatic effect was observed for cefoxitin, clarithromycin and azithromycin. Tigecycline showed the best antibacterial effect by decreasing the intracellular growth up to bactericidal level.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29626567</pmid><doi>10.1016/j.mimet.2018.03.020</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0167-7012
ispartof Journal of microbiological methods, 2018-05, Vol.148, p.29-32
issn 0167-7012
1872-8359
language eng
recordid cdi_proquest_miscellaneous_2022983293
source ScienceDirect Freedom Collection 2022-2024
subjects Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - pharmacokinetics
Anti-Bacterial Agents - pharmacology
Humans
Intracellular activity
Macrophages - metabolism
Macrophages - microbiology
Microbial Viability - drug effects
Models, Biological
Mycobacterium abscessus
Mycobacterium abscessus - drug effects
Mycobacterium abscessus - growth & development
Mycobacterium Infections, Nontuberculous - drug therapy
THP-1 Cells
THP-1 macrophages
title Evaluation of the intracellular activity of drugs against Mycobacterium abscessus using a THP-1 macrophage model
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T07%3A46%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20the%20intracellular%20activity%20of%20drugs%20against%20Mycobacterium%20abscessus%20using%20a%20THP-1%20macrophage%20model&rft.jtitle=Journal%20of%20microbiological%20methods&rft.au=Molina-Torres,%20Carmen%20Amelia&rft.date=2018-05&rft.volume=148&rft.spage=29&rft.epage=32&rft.pages=29-32&rft.issn=0167-7012&rft.eissn=1872-8359&rft_id=info:doi/10.1016/j.mimet.2018.03.020&rft_dat=%3Cproquest_cross%3E2022983293%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c359t-34dfb7ee186f0b80532ab2b15ec1d852433ed44c5726444d3d12383242b552f43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2022983293&rft_id=info:pmid/29626567&rfr_iscdi=true