NGS combined with phylogenetic analysis to detect HIV-1 dual infection in Romanian people who inject drugs

Dual HIV infections are possible and likely in people who inject drugs (PWID). Thirty-eight newly diagnosed patients, 19 PWID and 19 heterosexually HIV infected were analyzed. V2V3 loop of HIV-1 env gene was sequenced on the NGS platform 454 GSJunior (Roche). HIV-1 dual/multiple infections were iden...

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Bibliographic Details
Published in:Microbes and infection 2018-05, Vol.20 (5), p.308-311
Main Authors: Popescu, Bogdan, Banica, Leontina, Nicolae, Ionelia, Radu, Eugen, Niculescu, Iulia, Abagiu, Adrian, Otelea, Dan, Paraschiv, Simona
Format: Article
Language:English
Subjects:
HIV dual infection
Next generation sequencing
People who inject drugs
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Summary:Dual HIV infections are possible and likely in people who inject drugs (PWID). Thirty-eight newly diagnosed patients, 19 PWID and 19 heterosexually HIV infected were analyzed. V2V3 loop of HIV-1 env gene was sequenced on the NGS platform 454 GSJunior (Roche). HIV-1 dual/multiple infections were identified in five PWID. For three of these patients, the reconstructed variants belonged to pure F1 subtype and CRF14_BG strains according to phylogenetic analysis. New recombinant forms between these parental strains were identified in two PWID samples. NGS data can provide, with the help of phylogenetic analysis, important insights about the intra-host sub-population structure.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2018.03.004