Main Strategies of Plant Expression System Glycoengineering for Producing Humanized Recombinant Pharmaceutical Proteins

Most the pharmaceutical proteins are derived not from their natural sources, rather their recombinant analogs are synthesized in various expression systems. Plant expression systems, unlike mammalian cell cultures, combine simplicity and low cost of procaryotic systems and the ability for posttransl...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2018-03, Vol.83 (3), p.215-232
Main Authors: Rozov, S. M., Permyakova, N. V., Deineko, E. V.
Format: Article
Language:English
Subjects:
Biochemistry
Biological activity
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Biosynthesis
Gene expression
Glycoproteins
Glycosylation
Health aspects
Immunity
Immunogenicity
Life Sciences
Mammalian cells
Mammals
Microbiology
Pharmaceuticals
Pharmacodynamics
Pharmacokinetics
Pharmacology
Plant cells
Polysaccharides
Proteins
Recombinant proteins
Review
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Summary:Most the pharmaceutical proteins are derived not from their natural sources, rather their recombinant analogs are synthesized in various expression systems. Plant expression systems, unlike mammalian cell cultures, combine simplicity and low cost of procaryotic systems and the ability for posttranslational modifications inherent in eucaryotes. More than 50% of all human proteins and more than 40% of the currently used pharmaceutical proteins are glycosylated, that is, they are glycoproteins, and their biological activity, pharmacodynamics, and immunogenicity depend on the correct glycosylation pattern. This review examines in detail the similarities and differences between N- and O–glycosylation in plant and mammalian cells, as well as the effect of plant glycans on the activity, pharmacokinetics, immunity, and intensity of biosynthesis of pharmaceutical proteins. The main current strategies of glycoengineering of plant expression systems aimed at obtaining fully humanized proteins for pharmaceutical application are summarized.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297918030033