Long-lasting RNAi activity in mammalian neurons

The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21-nucleotide siRNA duplex in postmitotic neurons, mo...

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Bibliographic Details
Published in:FEBS letters 2004-01, Vol.558 (1), p.89-95
Main Authors: Omi, Kazuya, Tokunaga, Katsushi, Hohjoh, Hirohiko
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Cell Differentiation - drug effects
Cell Division
Cell Line, Tumor
Cells, Cultured
Cytarabine - pharmacology
dsRNA, double-stranded RNA
Gene Expression Profiling
Hippocampus - cytology
Hippocampus - embryology
Long double-stranded RNA
Mammalian neuron
Mice
Mice, Inbred ICR
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
nt, nucleotide
PKR, interferon-inducible, dsRNA-activated protein kinase
RNA interference
RNA, Double-Stranded - metabolism
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
RNAi, RNA interference
siRNA, small interfering RNA
Synthetic small interfering RNA
Teratocarcinoma - genetics
Teratocarcinoma - pathology
Time Factors
Tretinoin - pharmacology
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Summary:The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21-nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long-lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence-specific RNAi activity involving long dsRNA appears to be masked.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(04)00017-1