Biosynthesis of biodegradable polyhydroxyalkanotes biopolymers in genetically modified yeasts

In the recent decade, biosynthesis of the degradable biopolymers polyhydroxyalkanotes in transgenic yeasts became an important research task. Most research strategies depend on either metabolic engineering or molecular approaches. In the present work, research compared PHA biosynthesis in two types...

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Bibliographic Details
Published in:International journal of environmental science and technology (Tehran) 2007-09, Vol.4 (4), p.513-520
Main Authors: Abd-El-haleem, D, Amara, A, Zaki, S, Abulhamd, A, Abulreesh, G
Format: Article
Language:English
Subjects:
Biodegradable materials
Biopolymers
Kloeckera
NMR
Nuclear magnetic resonance
Ralstonia eutropha
Recombinant yeasts, polyhydroxyalkanotes, poly-3-hydroxybutyrate, nuclear magnetic resonance
Saccharomyces cerevisiae
Studies
Yeast
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Summary:In the recent decade, biosynthesis of the degradable biopolymers polyhydroxyalkanotes in transgenic yeasts became an important research task. Most research strategies depend on either metabolic engineering or molecular approaches. In the present work, research compared PHA biosynthesis in two types of yeasts; Saccharomyces cerevisiae and a non-convenient Kloeckera spp.Yeast strains were equipped in their cytoplasm with the phaABCRe operon containing genes phbA, phbB and phbC of the PHA biosynthetic pathway of Ralstonia eutropha , which encode â-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. The transgenic strains Saccharomyces cerevisiae and Kloeckera sp. were able to produce PHA. The maximum content of the polymer detected in the recombinant strain INVSc1/PHA1 was 2.68 % and only poly-3-hydroxybutyrate (PHB) accumulated. However, the non-conventional transgenic strain KY1/PHA was able to accumulate as maximum of 7.06 % of the copolymer poly-(3-hydroxybutyrateco-poly-3-hydroxyvalerate) (PHV). Western blot analysis confirmed expression of the phaABCReoperonin the transgenic yeast strains. The nature of the PHA thus produced by all tested strains was analyzed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy.
ISSN:1735-1472
1735-2630
DOI:10.1007/BF03325988