OCCURRENCE OF PSEUDOMONAS VIRIDIFLAVA ON TOMATO IN BRAZIL

During March to May 2008, a severe disease characterized by leaf necrosis was observed in Brazil on tomato cvs Saladinha, Debora Max and Carmen, grown in Mucuge (State of Bahia) and Apiai and Botucatu (State of Sao Paulo). Fluorescent, opaque and yellowish colonies of a rod-shaped Gram-negative bact...

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Bibliographic Details
Published in:Journal of plant pathology 2009-07, Vol.91 (2), p.504-504
Main Authors: Maringoni, A.C., Gioria, R., Kobori, R.F., Azevedo, S.M., De Pieri, C., Silva, T.A.F.
Format: Article
Language:English
Subjects:
Disease Note
Lycopersicon esculentum
Phytopathology
Plant diseases
Pseudomonas
Pseudomonas viridiflava
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Summary:During March to May 2008, a severe disease characterized by leaf necrosis was observed in Brazil on tomato cvs Saladinha, Debora Max and Carmen, grown in Mucuge (State of Bahia) and Apiai and Botucatu (State of Sao Paulo). Fluorescent, opaque and yellowish colonies of a rod-shaped Gram-negative bacterium were obtained on King' B medium following isolations from diseased tissue. Three bacterial strains were analysed with the MicroLog 4.2.05 System (Biolog, Hayward, USA) and identified as Pseudomonas viridiflava with a similarity index ranging from 66 to 83%. Biochemical and physiological characterization showed that all strains were aerobic, catalase positive, oxidase, levan, arginine dihydrolase, and nitrate reduction negative. They also showed pectinolitic activity, induced hypersensitive reactions in tobacco leaves, and produced acid from mannitol and sorbitol but not from sucrose. These results are in line with those reported for tomato strains of P. viridiflava (Goumas et al., 1999). For pathogenicity tests bacterial suspensions (10 super(7) CFU ml super(-1)) were sprayed on the leaves of tomato plants of cv. Santa Clara in which they induced necrotic spots within five days from inoculation. This was taken as evidence that the bacterial isolate used for inoculation is pathogenic to tomato. In Brazil, P. viridiflava has previously been reported a the agent of diseases of several cultivated plants (Malavolta Junior et al., 2008) but not of tomato. Thus, to the best of our knowledge, this is the first report of P. viridiflava infections to tomato in Brazil.
ISSN:1125-4653
2239-7264