Dual amplification strategy for improved efficiency of forensic DNA analysis using NGM Detect™, NGM™ or Globalfiler™ kits

•Optimal amount of DNA gave complete and concordant results when using NGM Detect™, NGM™ or GlobalFiler™ kits.•DNA analysis using the NGM Detect™ kit gives positive results for bone tissues where high DNA degradation is observed.•A dual amplification strategy with NGM Detect™ kit and the NGM™ and Gl...

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Bibliographic Details
Published in:Forensic science international : genetics 2018-07, Vol.35, p.46-49
Main Authors: Parys-Proszek, Agnieszka, Wróbel, Maria, Marcińska, Magdalena, Kupiec, Tomasz
Format: Article
Language:English
Subjects:
Blood Chemical Analysis
Bone and Bones - chemistry
DNA - isolation & purification
DNA Fingerprinting
Humans
Male
Microsatellite Repeats
miniSTRs
NGM detect
Polymerase Chain Reaction - instrumentation
Reduced-size STR amplicons
Saliva - chemistry
Semen - chemistry
Short tandem repeats
Tooth - chemistry
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Summary:•Optimal amount of DNA gave complete and concordant results when using NGM Detect™, NGM™ or GlobalFiler™ kits.•DNA analysis using the NGM Detect™ kit gives positive results for bone tissues where high DNA degradation is observed.•A dual amplification strategy with NGM Detect™ kit and the NGM™ and GlobalFiler™ kits increases the number of positively typed alleles. The new generation of STR amplification kits with improved sensitivity and additional genetic markers is designed particularly for analyzing difficult traces with a high DNA degradation index, presence of inhibitors and low level of DNA. In the new NGM Detect™ kit, modifications including changing the primers’ sequences and shortening of STR markers are introduced. The quality control system (IQCS, IQCL) used to detect DNA degradation and the presence of inhibitors in the sample is an additional feature. The purpose of this study was to compare the results of analysis of different kinds of typical casework samples obtained using NGM™ or GlobalFiler™ kits with those generated using the new NGM Detect™ kit. The results indicate that the tested kit is particularly useful for the analysis of challenging samples for which incomplete profiles are generated with the NGM™ or GlobalFiler™ kits. The increased number of positively typed alleles gives better statistical parameters in genetic identification cases. We conclude that the NGM Detect™ kit can be recommended for the double amplification protocol together with the NGM or GlobalFiler™ kits.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2018.03.018