Effects of basidiomycete laccase on cercosporin

Cercosporin is a perylenequinone pigment produced by fungi in the genus Cercospora which under light generates reactive oxygen species causing membrane damage and mortality of living cells. Our objectives were to evaluate the effects of laccase, a lignolitic copper-containing enzyme on the temporal...

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Bibliographic Details
Published in:Journal of plant pathology 2009-07, Vol.91 (2), p.347-355
Main Authors: Caesar-TonThat, T.C, Lartey, R.T, Solbert-Rodier, L, Caesar, A.J
Format: Article
Language:English
Subjects:
absorbance
airborne microorganisms
Basidiomycetes
Basidiomycota
Cercospora
Cercospora beticola
Clay soils
Coriolus versicolor
Dyes
Enzymes
fluorescence microscopy
Fungi
laccase
leaves
Lesions
mortality
Phytopathology
Pigments
plant tissues
Pleurotus
Pleurotus ostreatus
Reactive oxygen species
sugar beet
Sugar beets
toxicity
Trametes versicolor
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Summary:Cercosporin is a perylenequinone pigment produced by fungi in the genus Cercospora which under light generates reactive oxygen species causing membrane damage and mortality of living cells. Our objectives were to evaluate the effects of laccase, a lignolitic copper-containing enzyme on the temporal evolution of the UV-visible spectrum of cercosporin and to demonstrate that laccase can reduce cercosporin toxicity toward living cells. Cercosporin from Cercopsora beticola and Cercospora hayi was treated with laccase from basidiomycete fungi (Pleurotus ostreatus and Trametes versicolor) in the dark and under constant light. Under these conditions the absorbance of the cercosporin decreased at 220, 279 and 295-500 nm within 10 min of reaction with laccase from either P. ostreatus or T. versicolor, indicating that basidiomycete laccase can induce changes in UV-visible spectra of cercosporin. The LIVE/DEAD Bac Light VIABILITY kit and fluorescent microscopy showed more viable E. coli cells after incubation under light with cercosporin and laccase than with cercosporin alone. Lesions were apparent on sugar beet leaves exposed to cercosporin under light after 48 h, but leaves exposed to cercosporin and laccase showed visibly less damage. Furthermore, air-borne microorganisms can grow on cercosporin-producing C. beticola cultures with laccase added under light, but cercosporin-producing cultures without laccase appeared to inhibit their growth. These data suggests that laccase from basidiomycete fungi can decrease the toxic effect of cercosporin toward microorganisms and plant tissue.
ISSN:1125-4653
2239-7264