THE SIGNALING LIGAND STOMATIN TRIGGERS A RAPID INCREASE IN INTRACELLULAR CALCIUM CONCENTRATION IN TETRAHYMENA VORAX

The effect of the macrostomal-cell inducing ligand, stomatin, on intracellular calcium concentration in Tetrahymena vorax was examined using the cell permeable fluorescent indicator Calcium Orange/AM. Intracellular calcium concentration began to increase within approximately four minutes following s...

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Bibliographic Details
Published in:Florida scientist 2008-07, Vol.71 (3), p.287-292
Main Authors: Ryals, Phillip E., Damaso, Reynald M., Jones, Jessica B., Steinmetz, Christina A.
Format: Article
Language:English
Subjects:
Calcium
Cell membranes
Cells
Chemical suspensions
Emissions intensity
Fluorescence
Inositols
Ligands
Phenotypes
Phosphatidylinositols
Tetrahymena vorax
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Summary:The effect of the macrostomal-cell inducing ligand, stomatin, on intracellular calcium concentration in Tetrahymena vorax was examined using the cell permeable fluorescent indicator Calcium Orange/AM. Intracellular calcium concentration began to increase within approximately four minutes following stomatin addition to cells that had been pre-loaded with the calcium probe. Intracellular calcium levels continued to rise for at least 20 minutes. Cells incubated for 45 minutes with 100 μM aminoethoxydiphenylborate prior to the addition of stomatin showed no increase in intracellular calcium levels. These results, in combination with previously published data, suggest that the increase in calcium concentration in response to stomatin constitutes an important step in the transmembrane signaling pathway that leads to the development of the macrostomal phenotype in Tetrahymena vorax.
ISSN:0098-4590
DOI:10.1043/0098-4590(2008)71[287:TSLSTA]2.0.CO;2