Expression of DKKL1 in spermatozoa of men with asthenospermia

To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia. The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed usi...

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Bibliographic Details
Published in:Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2018-03, Vol.38 (3), p.324
Main Authors: Yan, Qiu-Xia, Ma, Yi, Chen, Run-Qiang, Zhou, Xiu-Qin, Qiao, Jing, Xian, Ying-Jie, Feng, Ling, Chen, Cai-Rong
Format: Article
Language:Chinese
Subjects:
Asthenozoospermia - metabolism
Blotting, Western
Case-Control Studies
Humans
Intercellular Signaling Peptides and Proteins - metabolism
Male
RNA, Messenger
Sperm Motility
Spermatozoa - metabolism
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Summary:To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia. The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting. The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P
ISSN:1673-4254