Analysis on sarcoglycans expression as markers of septic cardiomyopathy in sepsis-related death

• Published in: International journal of legal medicine 2018-11, Vol.132 (6), p.1685-1692
• Main Authors: Ventura Spagnolo, Elvira, Mondello, Cristina, Di Mauro, Debora, Vermiglio, Giovanna, Asmundo, Alessio, Filippini, Elena, Alibrandi, Angela, Rizzo, Giuseppina
• Format: Article
• Language: English
• Subjects: Aged; Autopsies; Biomarkers; Biomarkers - metabolism; Cardiomyopathies - metabolism; Cardiomyopathies - mortality; Cardiomyopathy; Case-Control Studies; Damage assessment; Death; Fatalities; Female; Fluorescence; Fluorescent Antibody Technique, Indirect; Forensic Medicine; Forensic Pathology; Heart; Humans; Immunofluorescence; Inflammatory response; Male; Markers; Medical Law; Medicine & Public Health; Mortality; Myocardium - metabolism; Original Article; Proteins; Retrospective Studies; Sarcoglycans - metabolism; Sarcopenia; Sepsis; Sepsis - metabolism; Sepsis - mortality; Staining
• ISSN: 0937-9827; 1437-1596
• DOI: 10.1007/s00414-018-1840-6

The post-mortem assessment of sepsis-related death can be carry out by many methods recently suggested as microbiological and biochemical investigations. In these cases, the cause of death is a multiple organ dysfunction due to a dysregulated inflammatory response occurring after the failure of infection control process. It was highlighted also that the heart can be a target organ in sepsis which determines the so-called septic cardiomyopathy characterized by myocardial depression. Several mechanisms to explain the pathophysiology of septic cardiomyopathy were suggested, but very few studies about the structural alterations of cardiac cells responsible for myocardial depression were carried out. The aim of this study was to evaluate whether sarcoglycans (SG) were involved in septic cardiac damage analyzing their expression in sepsis-related deaths and, particularly, if these proteins can be used as markers of septic myocardial dysfunction. Cases of septic-related death confirmed by clinical and autopsy records were investigated and compared to a control group of traumatic deaths. Indirect immunofluorescence analysis was performed to analyze α-SG, β-SG, δ-SG, ζ-SG, ε-SG, and γ-SG. Decrease of fluorescence staining pattern for all tested sarcoglycans was observed in the septic-related deaths compared to normal fluorescence staining pattern of control group. These results provide new findings about the myocytes structural alterations due to sepsis and suggest that these proteins could be used in forensic assessment of septic cardiomyopathy.