Plasma Xanthine Oxidoreductase Activity as a Novel Biomarker of Metabolic Disorders in a General Population

Background:Xanthine oxidoreductase (XOR) is an enzyme that catalyzes the formation of uric acid from hypoxanthine and xanthine, leading to an increase in superoxide and reactive oxygen species. Activation of XOR promotes oxidative stress-related tissue injury. We investigated the associations betwee...

Full description

Saved in:
Bibliographic Details
Published in:Circulation Journal 2018/06/25, Vol.82(7), pp.1892-1899
Main Authors: Furuhashi, Masato, Matsumoto, Megumi, Tanaka, Marenao, Moniwa, Norihito, Murase, Takayo, Nakamura, Takashi, Ohnishi, Hirofumi, Saitoh, Shigeyuki, Shimamoto, Kazuaki, Miura, Tetsuji
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Asian People
Biomarkers - blood
Cholesterol, HDL - blood
Chromatography, Liquid
Cohort Studies
Female
Humans
Insulin Resistance
Liver dysfunction
Male
Mass Spectrometry
Metabolic Diseases - diagnosis
Metabolic Diseases - enzymology
Metabolic Diseases - epidemiology
Middle Aged
Obesity
Smoking
Uric acid
Xanthine Dehydrogenase - blood
Xanthine Dehydrogenase - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background:Xanthine oxidoreductase (XOR) is an enzyme that catalyzes the formation of uric acid from hypoxanthine and xanthine, leading to an increase in superoxide and reactive oxygen species. Activation of XOR promotes oxidative stress-related tissue injury. We investigated the associations between metabolic parameters and plasma XOR activity measured by a sensitive and accurate assay using a combination of liquid chromatography and triple quadrupole mass spectrometry to detect [13C2,15N2]-uric acid using [13C2,15N2]-xanthine as a substrate.Methods and Results:A total of 627 Japanese subjects (M/F, 292/335) from the Tanno-Sobetsu Study, a population-based cohort, were recruited. Plasma XOR activity was significantly higher in males than in females, and habitual smoking was associated with elevation of activity. Plasma XOR activity was positively correlated with body mass index (BMI; r=0.323, P
ISSN:1346-9843
1347-4820
1347-4820
DOI:10.1253/circj.CJ-18-0082