In vitro quality of apheresis platelets divided into paediatric‐sized units and stored in PVC bags plasticised with TOTM, BTHC or DINCH

SUMMARY Background and Objectives Two of the predictive factors of the quality of small volumes of platelets suitable for paediatric use are bag size and material. This study evaluated the storage properties of paediatric platelet aliquots in TOTM‐, BTHC‐ or DINCH‐PVC bags. Methods/Materials (i) Thr...

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Bibliographic Details
Published in:Transfusion medicine (Oxford, England) England), 2018-10, Vol.28 (5), p.380-385
Main Authors: Bashir, S., Meli, A., Cardigan, R.
Format: Article
Language:English
Subjects:
Blood Platelets - cytology
Blood Platelets - metabolism
Blood Preservation - instrumentation
Blood Preservation - methods
Female
Humans
in vitro quality
Male
paediatric platelet concentrates
plasticiser
Plateletpheresis
Polyvinyl Chloride
storage bags
Time Factors
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Summary:SUMMARY Background and Objectives Two of the predictive factors of the quality of small volumes of platelets suitable for paediatric use are bag size and material. This study evaluated the storage properties of paediatric platelet aliquots in TOTM‐, BTHC‐ or DINCH‐PVC bags. Methods/Materials (i) Three apheresis platelet concentrates (PC) were pooled and split into three units. One was retained as an adult unit (control; polyolefin bag). The second and third units were split into four MacoPharma TOTM‐PVC and BTHC‐PVC paediatric bags, respectively. (ii) Two apheresis PC were pooled and split into two units. One PC was retained as an adult unit, and the other was split into four Fresenius DINCH‐PVC paediatric bags. Testing was performed on storage for pH, blood gases, hypotonic shock response, soluble CD62P, LDH, glucose and lactate, ATP, CD62P, CD63, platelet‐derived microparticles and annexin V. Results The volumes, platelet yields and pH of all paediatric units met local specifications. The TOTM‐PVC bag showed no worse quality than the adult bag up to day 7 for all parameters studied, and it maintained pH higher than BTHC‐PVC and DINCH‐PVC over storage. The BTHC‐PVC bag was shown to be the most gas permeable; however, it had the highest glucose consumption rates and the highest platelet activation. Conclusion All bags showed an acceptable in vitro quality. Overall, the TOTM‐PVC paediatric bag showed better platelet quality compared to the other storage bags, whereas storage in the BTHC‐PVC bag resulted in poorer platelet quality.
ISSN:0958-7578
1365-3148
DOI:10.1111/tme.12528