A Dual Alkylated Peptide-ligand Enhances Affinity to Human Serum Albumin

Therapeutic peptides and diagnostic agents with their molecular size below the renal clearance threshold suffer from short blood circulation time. Here, we report a novel design of peptide-based ligand with a strong binding affinity to human serum albumin (HSA), which can be used as a tag to extend...

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Bibliographic Details
Published in:Analytical Sciences 2018/04/10, Vol.34(4), pp.501-504
Main Authors: NAKHAEI, Elnaz, TAKEHARA, Ko, SATO, Hikari, ZAI, Khadijah, KISHIMURA, Akihiro, MORI, Takeshi, KATAYAMA, Yoshiki
Format: Article
Language:English
Subjects:
Affinity
Albumin
albumin-binding
Alkylation
Analytical Chemistry
ANS
Binding
Blood circulation
Chemical compounds
Chemistry
Diagnostic agents
Diagnostic systems
half-life extension
Human serum albumin
Human serum albumin (HSA)
Ligands
Molecular chains
Peptides
Pharmacology
Serum albumin
Spacer
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Summary:Therapeutic peptides and diagnostic agents with their molecular size below the renal clearance threshold suffer from short blood circulation time. Here, we report a novel design of peptide-based ligand with a strong binding affinity to human serum albumin (HSA), which can be used as a tag to extend the blood circulation of small-size molecules. We designed ligands with dual alkyl groups connected with a negatively charged spacer. The ligands showed both higher binding affinity to HSA and a higher retention in mice blood than that of a single alkylated peptide.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.17P614