Novel Toll-Like Receptor 9 Agonist Induces Epidermal Growth Factor Receptor (EGFR) Inhibition and Synergistic Antitumor Activity with EGFR Inhibitors

• Published in: Clinical cancer research 2006-01, Vol.12 (2), p.577-583
• Main Authors: DAMIANO, Vincenzo, CAPUTO, Rosa, BIANCO, Roberto, D'ARMIENTO, Francesco P, LEONARDI, Antonio, DE PLACIDO, Sabino, BIANCO, A. Raffaele, AGRAWAL, Sudhir, CIARDIELLO, Fortunato, TORTORA, Giampaolo
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - therapeutic use; Antibodies, Monoclonal, Humanized; Antineoplastic agents; Antineoplastic Agents - therapeutic use; Biological and medical sciences; biological therapeutic agents; Cell Proliferation - drug effects; Cetuximab; Colonic Neoplasms - drug therapy; Colonic Neoplasms - metabolism; Colonic Neoplasms - pathology; Cyclooxygenase 2 - metabolism; Drug Resistance, Neoplasm; Drug Synergism; Drug Therapy, Combination; EGFR; growth factor receptors and other surface molecules as targets for therapy; Humans; mechanisms of drug action/new molecular targets/therapeutics; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Nude; Mitogen-Activated Protein Kinases - metabolism; Neovascularization, Pathologic - prevention & control; NF-kappa B - genetics; NF-kappa B - metabolism; Oligonucleotides - therapeutic use; oligonucleotides; cancer therapy; Pharmacology. Drug treatments; Proto-Oncogene Proteins c-akt - metabolism; Proto-Oncogene Proteins c-bcl-2 - metabolism; Receptor, Epidermal Growth Factor - antagonists & inhibitors; Receptor, Epidermal Growth Factor - immunology; Signal Transduction - drug effects; Toll-Like Receptor 9 - agonists; Transplantation, Heterologous; Vascular Endothelial Growth Factor A
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.CCR-05-1943

Purpose: Immunostimulating Toll-like receptor 9 (TLR9) agonists cause antitumor activity interfering also with cancer proliferation and angiogenesis by mechanisms still incompletely understood. We hypothesized that modified TLR9 agonists could impair epidermal growth factor receptor (EGFR) signaling and, by this means, greatly enhance EGFR inhibitors effect, acting on both the receptor targeting and the immunologic arm. Experimental Design: We used a novel second-generation, modified, immunomodulatory TLR9 agonist (IMO), alone and in combination with the anti-EGFR monoclonal antibody cetuximab or tyrosine kinase inhibitor gefitinib, on the growth of GEO and cetuximab-resistant derivatives GEO-CR colon cancer xenografts. We have also evaluated the expression of several proteins critical for cell proliferation, apoptosis, and angiogenesis, including EGFR, mitogen-activated protein kinase, Akt, bcl-2, cyclooxygenase-2, vascular endothelial growth factor, and nuclear factor-κB. Results: IMO inhibited GEO growth and signaling by EGFR and the other proteins critical for cell proliferation and angiogenesis. IMO plus the anti-EGFR antibody cetuximab synergistically inhibited tumor growth, signaling proteins, and microvessel formation. EGFR signaling inhibition by IMO is relevant because IMO cooperated also with EGFR tyrosine kinase inhibitor gefitinib in GEO tumors, while it was inactive against GEO-CR xenografts. On the other hand, IMO boosted the non-EGFR-dependent cetuximab activity, causing a cooperative antitumor effect in GEO-CR cells. Finally, combination of IMO, cetuximab and chemotherapeutic irinotecan eradicated the tumors in 90% of mice. Conclusion: IMO interferes with EGFR-related signaling and angiogenesis and has a synergistic antitumor effect with EGFR inhibitors, especially with cetuximab, boosting both the EGFR dependent and independent activity of this agent. Moreover, this therapeutic strategy could be translated in patients affected by colorectal cancer.