Comparative transcriptome analysis of arsenate and arsenite stresses in rice seedlings

• Published in: Chemosphere (Oxford) 2009-02, Vol.74 (5), p.688-702
• Main Authors: Chakrabarty, Debasis, Trivedi, Prabodh Kumar, Misra, Prashant, Tiwari, Manish, Shri, Manju, Shukla, Devesh, Kumar, Smita, Rai, Arti, Pandey, Ashutosh, Nigam, Deepti, Tripathi, Rudra Dev, Tuli, Rakesh
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Applied ecology; arsenates; Arsenates - pharmacology; Arsenic; arsenites; Arsenites - pharmacology; Biological and medical sciences; cadmium; chromium; cytochrome P-450; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; gene expression regulation; General aspects; Genes, Plant; Genome, Plant; Genome-wide expression; glutaredoxin; glutathione; Glutathione metabolism; heat shock proteins; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Herbicides - pharmacology; lead; Metabolic network; metabolism; Microarray; microarray technology; Oryza - drug effects; Oryza - genetics; Oryza - metabolism; Oryza sativa; Plant Proteins - genetics; Plant Proteins - metabolism; plant stress; Reverse Transcriptase Polymerase Chain Reaction; rice; seedlings; Seedlings - drug effects; Seedlings - genetics; Seedlings - growth & development; soil pollution; Stress, Physiological; sulfates; Techniques; transcriptome; transcriptome analysis; transporters
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2008.09.082

The effect of arsenic (As) exposure on genome-wide expression was examined in rice ( Oryza sativa L., ssp . Indica). A group of defense and stress-responsive genes, transporters, heat-shock proteins, metallothioneins, sulfate-metabolizing proteins, and regulatory genes showed differential expression in rice seedlings challenged with arsenate (AsV) and arsenite (AsIII). AsV stress led to upregulation or downregulation of an additional set of genes in comparison to AsIII. Differential expression of several genes that showed the highest contrast in a microarray analysis was validated by following the quantitative changes in the levels of individual transcripts following challenge with AsV, AsIII, Cd, Cr, and Pb. Most of the selected genes responded to challenge by heavy metals such as arsenic. However, expression of one of the cytochrome P450 genes (Os01g43740) in rice root was induced by AsV but not by other heavy metals. Similarly, one glutaredoxin (Os01g26912) is expressed specifically in the AsIII-treated shoot.