Purification, partial characterization and inducing tumor cell apoptosis activity of a polysaccharide from Ganoderma applanatum

In this study, a homogeneous polysaccharide (designated as GAP-3S) with an average molecular weight of 6.82×105 Da was purified from Ganoderma applanatum. GAP-3S was composed of glucose, galactose, fucose and xylose in the molar ratio of 7.1:2.6:1.1:1. It could significantly inhibit the proliferatio...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2018-08, Vol.115, p.10-17
Main Authors: Zhen, Dong, Su, Ling, Miao, Yue, Zhao, Fei, Ren, Guihong, Mahfuz, Shad, Song, Hui
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, a homogeneous polysaccharide (designated as GAP-3S) with an average molecular weight of 6.82×105 Da was purified from Ganoderma applanatum. GAP-3S was composed of glucose, galactose, fucose and xylose in the molar ratio of 7.1:2.6:1.1:1. It could significantly inhibit the proliferation of MCF-7 cells in a dose- and time-dependent manner. Flow cytometry analysis indicated that GAP-3S could also induce cell apoptosis. Treatment of MCF-7 cells with GAP-3S lead to the collapse of mitochondrial membrane potential and the accumulation of ROS, up-regulated expression of Bax, cleavage of PARP, p-p38 and p-JNK, and the down-regulated expression of Bcl-2, PARP and p-ERK. The activities of caspase-3 and caspase-9 were also increased in GAP-3S-treated MCF-7 cells compared to untreated cells. These findings suggested that GAP-3S probably induced apoptosis in MCF-7 cells through intrinsic mitochondrial apoptosis and MAPK signaling pathways.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2018.03.062