Effect of Oxidized Dextran on Cytokine Production and Activation of IRF3 Transcription Factor in Macrophages from Mice of Opposite Strains with Different Sensitivity to Tuberculosis Infection

• Published in: Bulletin of experimental biology and medicine 2018-04, Vol.164 (6), p.738-742
• Main Authors: Chechushkov, A. V., Kozhin, P. M., Zaitseva, N. S., Gainutdinov, P. I., Men’shchikova, E. B., Troitskii, A. V., Shkurupy, V. A.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Cell activation; Cell Biology; Cytokines; Dextran; Dextrans; Health aspects; Inflammation; Interferon regulatory factor 3; Interleukin 10; Interleukin 12; Internal Medicine; Laboratory Medicine; Lipopolysaccharides; Macrophages; Microbiology and Immunology; Monocyte chemoattractant protein 1; Pathology; Peritoneum; Phenotypes; Rodents; Transcription activation; Transcription factors; Tuberculosis; Tumor necrosis factor; Tumor necrosis factor-α
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-018-4070-5

We studied differences in the production of pro- and anti-inflammatory cytokines and IRF3 transcription factor by peritoneal macrophages from mice of opposite strains CBA/J and C57Bl/6 and the effect of 60-kDa oxidized dextran on these parameters. Macrophages from C57Bl/6 mice were mainly characterized by the production of proinflammatory cytokines TNFα, IL-12, and MCP-1 (markers of M1 polarization). By contrast, CBA/J mice exhibited a relatively high level of anti-inflammatory cytokine IL-10 and lower expression of proinflammatory cytokines (M2 phenotype). IRF3 content in peritoneal macrophages of CBA/J mice was higher than in C57Bl/6 mice. Oxidized dextran decreased the expression of IRF3 upon stimulation of cells from CBA/J mice with LPS, but increased this process in C57Bl/6 mice. Despite a diversity of oxidized dextran-induced changes in cytokine production, the data confirm our hypothesis that this agent can stimulate the alternative activation of macrophages.