Laminin-1 acts as an adhesive for odontoblast-like cells and promotes their differentiation toward a hard tissue-forming phenotype

The present study was designed to investigate the effect of laminin-1 (LN-1 or LN-111) on an odontoblast-like cell line, MDPC-23. Wells of non-treated polystyrene plates were coated with various concentrations of LN-1 (0.1, 1, 10, and 100 µg/mL) and left to dry for 2 days. Water-coated surfaces were...

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Bibliographic Details
Published in:Journal of Oral Science 2018, Vol.60(2), pp.253-261
Main Authors: Tang, Jia, Saito, Takashi
Format: Article
Language:English
Subjects:
adhesive
Alkaline Phosphatase - metabolism
Animals
Anthraquinones
Cell Differentiation
Cell Line
Cell Proliferation
Coloring Agents
Dentistry
hard tissue
Humans
laminin
Laminin - physiology
Mice
odontoblast-like cell
Odontoblasts - cytology
Odontoblasts - enzymology
Odontoblasts - metabolism
Real-Time Polymerase Chain Reaction
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Summary:The present study was designed to investigate the effect of laminin-1 (LN-1 or LN-111) on an odontoblast-like cell line, MDPC-23. Wells of non-treated polystyrene plates were coated with various concentrations of LN-1 (0.1, 1, 10, and 100 µg/mL) and left to dry for 2 days. Water-coated surfaces were used as controls. MDPC-23 cell proliferation, differentiation and mineralization were evaluated in terms of the CCK-8 assay, ALP activity, real-time RT-PCR and Alizarin red staining. The data indicated that LN-1 promoted the proliferation of MDPC-23 cells in a concentration-dependent manner. Moreover, it enhanced ALP activity and expression of key odontogenic genes (DMP-1 and DSPP) upon addition of mineralization reagents, leading to significant promotion of calcification by the cells. These results demonstrate that LN-1 acts as an adhesive for odontoblast-like cells, allowing up-regulation of odontogenic genes and accelerating matrix mineralization. In the context of the present study, the optimal LN-1 coating concentration for MDPC-23 cells was suggested to be 100 µg/mL.
ISSN:1343-4934
1880-4926
DOI:10.2334/josnusd.17-0286