Modeling of 3D Structure of Chimeric Constructs Based on Hemagglutinin of Influenza Virus and Immunogenic Epitopes of Streptococcus Agalactiae

A project of an experimental recombinant vector vaccine for prevention of diseases caused by pathogenic streptococci based on ScaAB lipoprotein of Streptococcus agalactiae and a coldadapted strain of live influenza vaccine as a vector was developed. The sequence of ScaAB lipoprotein was analyzed and...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2018-04, Vol.164 (6), p.743-748
Main Authors: Fedorova, E. A., Smolonogina, T. A., Isakova-Sivak, I. N., Koren’kov, D. A., Kotomina, T. S., Leont’eva, G. F., Suvorov, A. N., Rudenko, L. G.
Format: Article
Language:English
Subjects:
Analysis
Antigenic determinants
Biomedical and Life Sciences
Biomedicine
Cell Biology
Epitopes
Genetic vectors
Hemagglutinins
Immunogenicity
Influenza
Influenza vaccines
Influenza viruses
Internal Medicine
Laboratory Medicine
Lectins
Lipoproteins
Pathology
Prevention
Streptococcus
Streptococcus infections
Vaccines
Virology
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Summary:A project of an experimental recombinant vector vaccine for prevention of diseases caused by pathogenic streptococci based on ScaAB lipoprotein of Streptococcus agalactiae and a coldadapted strain of live influenza vaccine as a vector was developed. The sequence of ScaAB lipoprotein was analyzed and fragments forming immunodominant epitopes were determined. Chimeric molecules of influenza virus hemagglutinin H7 carrying insertions of bacterial origin were constructed. Based on the results of simulation, the most promising variants were selected; they represented fragments of lipoprotein ScaAB lacking N-terminal domain bound to hemagglutinin via a flexible linker. These insertions should minimally modulate the properties of the influenza strain, while retaining potential immunogenicity to a wide group of pathogenic streptococci.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-018-4071-4