Resistance selection and biochemical mechanism of resistance to two Acaricides in Tetranychus cinnabarinus (Boiduval)

A Tetranychus cinnabarinus strain was collected from Chongqing, China. After 42 generations of selection with abamectin and 20 generations of selection with fenpropathrin in the laboratory, this T. cinnabarinus strain developed 8.7- and 28.7-fold resistance, respectively. Resistance to abamectin in...

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Bibliographic Details
Published in:Pesticide biochemistry and physiology 2009, Vol.93 (1), p.47-52
Main Authors: Lin, He, Chuan-hua, Xue, Jin-jun, Wang, Ming, Li, Wen-cai, Lu, Zhi-mo, Zhao
Format: Article
Language:English
Subjects:
Abamectin
acaricide resistance
Biological and medical sciences
Detoxification enzyme
enzyme activity
enzyme inhibitors
Fenpropathrin
Fundamental and applied biological sciences. Psychology
mites
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
Resistance
strains
T. cinnabarinus
Tetranychus cinnabarinus
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Summary:A Tetranychus cinnabarinus strain was collected from Chongqing, China. After 42 generations of selection with abamectin and 20 generations of selection with fenpropathrin in the laboratory, this T. cinnabarinus strain developed 8.7- and 28.7-fold resistance, respectively. Resistance to abamectin in AbR (abamectin resistant strain) and to fenpropathrin in FeR (fenpropathrin resistant strain) was partially suppressed by piperonyl butoxide (PBO), diethyl maleate (DEM) and triphenyl phosphate (TPP), inhibitors of mixed function oxidase (MFO), glutathione S-transferases (GST), and hydrolases, respectively, suggesting that these three enzyme families are important in conferring abamectin and fenpropathrin resistance in T. cinnabarinus. The major resistant mechanism to abamectin was the increasing activities of carboxylesterases (CarE), glutathione- S-transferase (GST) and mixed function oxidase (MFO), and the activity in resistant strain developed 2.7-, 3.4- and 1.4-fold contrasted to that in susceptible strain, respectively. The activity of glutathione- S-transferase (GST) in the FeR strain developed 2.8-fold when compared with the susceptible strain, which meant the resistance to fenpropathrin was related with the activity increase of glutathione- S-transferase (GST) in T. cinnabarinus. The result of the kinetic mensuration of carboxylesterases (CarE) showed that the structure of CarE in the AbR has been changed.
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2008.11.001