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Antibiotic resistance profiles of coagulase-positive and coagulase-negative staphylococci from pit latrine fecal sludge in a peri-urban South African community
The aim of this study was to assess pit latrine samples from a peri-urban community in KwaZulu-Natal (South Africa) for the presence of multidrug-resistant (MDR) Staphylococcus spp. Standard procedures were used to isolate Staphylococcus spp. from pit latrine fecal sludge samples, with confirmation...
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| Published in: | Folia microbiologica 2018-09, Vol.63 (5), p.645-651 |
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| cites | cdi_FETCH-LOGICAL-c372t-8ddd7ca6d4e801b52386766a749b731637860a3926b5650ce5541bb59188f3fa3 |
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| container_title | Folia microbiologica |
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| creator | Beukes, Lorika S. Schmidt, Stefan |
| description | The aim of this study was to assess pit latrine samples from a peri-urban community in KwaZulu-Natal (South Africa) for the presence of multidrug-resistant (MDR)
Staphylococcus
spp. Standard procedures were used to isolate
Staphylococcus
spp. from pit latrine fecal sludge samples, with confirmation at genus level by polymerase chain reaction (PCR). Sixty-eight randomly selected pit latrine
Staphylococcus
spp. isolates were further characterized by using established disk diffusion procedures. An average
Staphylococcus
spp. count of 2.1 × 10
5
CFU per g fecal material was established using two randomly selected pit latrine samples. Of the 68-selected
Staphylococcus
spp. pit latrine isolates, 49% were identified as coagulase positive, 51% as coagulase negative and 65% (12 coagulase positive, 32 coagulase negative isolates) were categorized as MDR. The majority (66/68) of
Staphylococcus
spp. isolates displayed resistance to fusidic acid while only 5/68 isolates displayed resistance to chloramphenicol. The pit latrine samples analyzed in this study are a source of MDR
Staphylococcus
spp., highlighting the need for proper hygiene and sanitation regimes in rural communities using these facilities. |
| doi_str_mv | 10.1007/s12223-018-0605-4 |
| format | article |
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Staphylococcus
spp. Standard procedures were used to isolate
Staphylococcus
spp. from pit latrine fecal sludge samples, with confirmation at genus level by polymerase chain reaction (PCR). Sixty-eight randomly selected pit latrine
Staphylococcus
spp. isolates were further characterized by using established disk diffusion procedures. An average
Staphylococcus
spp. count of 2.1 × 10
5
CFU per g fecal material was established using two randomly selected pit latrine samples. Of the 68-selected
Staphylococcus
spp. pit latrine isolates, 49% were identified as coagulase positive, 51% as coagulase negative and 65% (12 coagulase positive, 32 coagulase negative isolates) were categorized as MDR. The majority (66/68) of
Staphylococcus
spp. isolates displayed resistance to fusidic acid while only 5/68 isolates displayed resistance to chloramphenicol. The pit latrine samples analyzed in this study are a source of MDR
Staphylococcus
spp., highlighting the need for proper hygiene and sanitation regimes in rural communities using these facilities.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/s12223-018-0605-4</identifier><identifier>PMID: 29663126</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acid resistance ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; Applied Microbiology ; Bacterial Proteins - metabolism ; Biomedical and Life Sciences ; Chloramphenicol ; Coagulase ; Coagulase - metabolism ; DNA, Bacterial - genetics ; Drug Resistance, Multiple, Bacterial ; Environmental Engineering/Biotechnology ; Feces ; Feces - microbiology ; Fusidic acid ; Genes, Bacterial - genetics ; Hygiene ; Immunology ; Life Sciences ; Microbial Sensitivity Tests ; Microbial Viability - drug effects ; Microbiology ; Multidrug resistance ; Original Article ; Polymerase chain reaction ; Poverty ; Rural areas ; Rural communities ; Sanitation ; Sewage - microbiology ; Sludge ; South Africa ; Staphylococcus ; Staphylococcus - drug effects ; Staphylococcus - enzymology ; Staphylococcus - genetics ; Staphylococcus - isolation & purification ; Toilet Facilities ; Urban areas</subject><ispartof>Folia microbiologica, 2018-09, Vol.63 (5), p.645-651</ispartof><rights>Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2018</rights><rights>Folia Microbiologica is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-8ddd7ca6d4e801b52386766a749b731637860a3926b5650ce5541bb59188f3fa3</citedby><cites>FETCH-LOGICAL-c372t-8ddd7ca6d4e801b52386766a749b731637860a3926b5650ce5541bb59188f3fa3</cites><orcidid>0000-0001-5584-1681</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29663126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beukes, Lorika S.</creatorcontrib><creatorcontrib>Schmidt, Stefan</creatorcontrib><title>Antibiotic resistance profiles of coagulase-positive and coagulase-negative staphylococci from pit latrine fecal sludge in a peri-urban South African community</title><title>Folia microbiologica</title><addtitle>Folia Microbiol</addtitle><addtitle>Folia Microbiol (Praha)</addtitle><description>The aim of this study was to assess pit latrine samples from a peri-urban community in KwaZulu-Natal (South Africa) for the presence of multidrug-resistant (MDR)
Staphylococcus
spp. Standard procedures were used to isolate
Staphylococcus
spp. from pit latrine fecal sludge samples, with confirmation at genus level by polymerase chain reaction (PCR). Sixty-eight randomly selected pit latrine
Staphylococcus
spp. isolates were further characterized by using established disk diffusion procedures. An average
Staphylococcus
spp. count of 2.1 × 10
5
CFU per g fecal material was established using two randomly selected pit latrine samples. Of the 68-selected
Staphylococcus
spp. pit latrine isolates, 49% were identified as coagulase positive, 51% as coagulase negative and 65% (12 coagulase positive, 32 coagulase negative isolates) were categorized as MDR. The majority (66/68) of
Staphylococcus
spp. isolates displayed resistance to fusidic acid while only 5/68 isolates displayed resistance to chloramphenicol. The pit latrine samples analyzed in this study are a source of MDR
Staphylococcus
spp., highlighting the need for proper hygiene and sanitation regimes in rural communities using these facilities.</description><subject>Acid resistance</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Applied Microbiology</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Chloramphenicol</subject><subject>Coagulase</subject><subject>Coagulase - metabolism</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>Fusidic acid</subject><subject>Genes, Bacterial - genetics</subject><subject>Hygiene</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbial Viability - drug effects</subject><subject>Microbiology</subject><subject>Multidrug resistance</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Poverty</subject><subject>Rural areas</subject><subject>Rural communities</subject><subject>Sanitation</subject><subject>Sewage - microbiology</subject><subject>Sludge</subject><subject>South Africa</subject><subject>Staphylococcus</subject><subject>Staphylococcus - drug effects</subject><subject>Staphylococcus - enzymology</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - isolation & purification</subject><subject>Toilet Facilities</subject><subject>Urban areas</subject><issn>0015-5632</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kcuKFDEUhoMoTjv6AG4k4MZNNJdKUrVshvECAy7UdZFKnfRkqErKXIR-Gl_VtD1eEFyFnHz_n4QPoeeMvmaU6jeZcc4FoawnVFFJugdox3rdkUFI9RDtKGWSSCX4BXqS8x1tUCf4Y3TBB6UE42qHvu9D8ZOPxVucIPtcTLCAtxSdXyDj6LCN5lAXk4FsMfvivwE2Yf5rHOBgfo5beLs9LtFGaz12Ka548wUvpiQfADuwZsF5qfMBsA_Y4A2SJzVNJuBPsZZbvHfJ27azcV1r8OX4FD1yZsnw7H69RF_eXn--ek9uPr77cLW_IVZoXkg_z7O2Rs0d9JRNkoteaaWM7oZJC6aE7hU1YuBqkkpSC1J2bJrkwPreCWfEJXp17m0__1ohl3H12cKymACx5pFTrrpTj2zoy3_Qu1hTaK87UVJSPQjeKHambIo5J3Djlvxq0nFkdDzZG8_2xmZvPNkbu5Z5cd9cpxXm34lfuhrAz0BuR-EA6c_V_2_9Ab_xpwk</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Beukes, Lorika S.</creator><creator>Schmidt, Stefan</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5584-1681</orcidid></search><sort><creationdate>20180901</creationdate><title>Antibiotic resistance profiles of coagulase-positive and coagulase-negative staphylococci from pit latrine fecal sludge in a peri-urban South African community</title><author>Beukes, Lorika S. ; Schmidt, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-8ddd7ca6d4e801b52386766a749b731637860a3926b5650ce5541bb59188f3fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acid resistance</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Applied Microbiology</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Chloramphenicol</topic><topic>Coagulase</topic><topic>Coagulase - metabolism</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>Fusidic acid</topic><topic>Genes, Bacterial - genetics</topic><topic>Hygiene</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbial Viability - drug effects</topic><topic>Microbiology</topic><topic>Multidrug resistance</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Poverty</topic><topic>Rural areas</topic><topic>Rural communities</topic><topic>Sanitation</topic><topic>Sewage - microbiology</topic><topic>Sludge</topic><topic>South Africa</topic><topic>Staphylococcus</topic><topic>Staphylococcus - drug effects</topic><topic>Staphylococcus - enzymology</topic><topic>Staphylococcus - genetics</topic><topic>Staphylococcus - isolation & purification</topic><topic>Toilet Facilities</topic><topic>Urban areas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beukes, Lorika S.</creatorcontrib><creatorcontrib>Schmidt, Stefan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beukes, Lorika S.</au><au>Schmidt, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibiotic resistance profiles of coagulase-positive and coagulase-negative staphylococci from pit latrine fecal sludge in a peri-urban South African community</atitle><jtitle>Folia microbiologica</jtitle><stitle>Folia Microbiol</stitle><addtitle>Folia Microbiol (Praha)</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>63</volume><issue>5</issue><spage>645</spage><epage>651</epage><pages>645-651</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><abstract>The aim of this study was to assess pit latrine samples from a peri-urban community in KwaZulu-Natal (South Africa) for the presence of multidrug-resistant (MDR)
Staphylococcus
spp. Standard procedures were used to isolate
Staphylococcus
spp. from pit latrine fecal sludge samples, with confirmation at genus level by polymerase chain reaction (PCR). Sixty-eight randomly selected pit latrine
Staphylococcus
spp. isolates were further characterized by using established disk diffusion procedures. An average
Staphylococcus
spp. count of 2.1 × 10
5
CFU per g fecal material was established using two randomly selected pit latrine samples. Of the 68-selected
Staphylococcus
spp. pit latrine isolates, 49% were identified as coagulase positive, 51% as coagulase negative and 65% (12 coagulase positive, 32 coagulase negative isolates) were categorized as MDR. The majority (66/68) of
Staphylococcus
spp. isolates displayed resistance to fusidic acid while only 5/68 isolates displayed resistance to chloramphenicol. The pit latrine samples analyzed in this study are a source of MDR
Staphylococcus
spp., highlighting the need for proper hygiene and sanitation regimes in rural communities using these facilities.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29663126</pmid><doi>10.1007/s12223-018-0605-4</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-5584-1681</orcidid></addata></record> |
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| language | eng |
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| subjects | Acid resistance Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Applied Microbiology Bacterial Proteins - metabolism Biomedical and Life Sciences Chloramphenicol Coagulase Coagulase - metabolism DNA, Bacterial - genetics Drug Resistance, Multiple, Bacterial Environmental Engineering/Biotechnology Feces Feces - microbiology Fusidic acid Genes, Bacterial - genetics Hygiene Immunology Life Sciences Microbial Sensitivity Tests Microbial Viability - drug effects Microbiology Multidrug resistance Original Article Polymerase chain reaction Poverty Rural areas Rural communities Sanitation Sewage - microbiology Sludge South Africa Staphylococcus Staphylococcus - drug effects Staphylococcus - enzymology Staphylococcus - genetics Staphylococcus - isolation & purification Toilet Facilities Urban areas |
| title | Antibiotic resistance profiles of coagulase-positive and coagulase-negative staphylococci from pit latrine fecal sludge in a peri-urban South African community |
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