Chloroplast-localized nonspecific lipid transfer protein with anti-fungal activity from rough lemon

A full-length cDNA ( RlemLTP) from rough lemon that encodes a nonspecific lipid transfer protein was isolated. Results of subcellular localization of RlemLTP fused to a green fluorescence protein indicated that RlemLTP is located in the chloroplast. Transcripts of RlemLTP were detected in leaves and...

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Bibliographic Details
Published in:Physiological and molecular plant pathology 2008-07, Vol.72 (4), p.134-140
Main Authors: Nishimura, Satoshi, Tatano, Satoshi, Gomi, Kenji, Ohtani, Kouhei, Fukumoto, Takeshi, Akimitsu, Kazuya
Format: Article
Language:English
Subjects:
Alternaria alternata
Anti-fungal protein
Biological and medical sciences
Citrus jambhiri
Escherichia coli
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Fusarium oxysporum
Fusarium oxysporum f. sp. lycopersici
Gene expression
Genes. Genome
Molecular and cellular biology
Molecular genetics
Nonspecific lipid transfer protein
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
Phytopathology. Animal pests. Plant and forest protection
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Summary:A full-length cDNA ( RlemLTP) from rough lemon that encodes a nonspecific lipid transfer protein was isolated. Results of subcellular localization of RlemLTP fused to a green fluorescence protein indicated that RlemLTP is located in the chloroplast. Transcripts of RlemLTP were detected in leaves and seeds but not in stems and roots. The transcription of RlemLTP was higher in young leaves than in old leaves. Wounding and fungal inoculation induced RlemLTP expression, and transcription levels were increased 6–15 times by these treatments. RlemLTP obtained using an E. coli expression system showed anti-fungal activity against Alternaria alternata and Fusarium oxysporum.
ISSN:0885-5765
1096-1178
DOI:10.1016/j.pmpp.2008.07.003