Experimental study of the evanescent‐wave photonic sensors response in presence of molecular beacon conformational changes

An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes—upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors—is reported. To this end, high sensitivity photonic sensors based on silicon photonic ban...

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Bibliographic Details
Published in:Journal of biophotonics 2018-10, Vol.11 (10), p.e201800030-n/a
Main Authors: Ruiz‐Tórtola, Ángela, Prats‐Quílez, Francisco, González‐Lucas, Daniel, Bañuls, María‐José, Maquieira, Ángel, Wheeler, Guy, Dalmay, Tamas, Griol, Amadeu, Hurtado, Juan, Bohlmann, Helge, Götzen, Reiner, García‐Rupérez, Jaime
Format: Article
Language:English
Subjects:
Biotin
conformational change
Evanescent waves
Hybridization
molecular beacon
Nucleic acids
Oligonucleotides
Photonic band gaps
photonic bandgap
photonic biosensor
Photonics
Probes
Sensors
silicon photonics
Streptavidin
Target detection
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Summary:An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes—upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors—is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5′ termination. Those MBs incorporate a biotin moiety close to their 3′ termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure. Recent results have proven the high potential of molecular beacon (MB) probes for the recognition of short sequence nucleic acid strands in solution. Likewise, nanophotonic technology is a promising candidate for the development of high‐throughput and high‐sensitivity biosensors. On this basis, the MB conformation change upon hybridization is exploited to experimentally study the interaction between molecule‐labeled MBs and evanescent‐wave photonic sensors.
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201800030