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Human papillomavirus and mutated H-ras oncogene in cervical carcinomas and pathological negative pelvic lymph nodes: A retrospective follow-up

The metastasis status of pelvic lymph nodes (PLNs) seems to be a predictive factor of survival. It was suggested that the presence of HPV DNA and other biological markers in PLN may indicate a sub clinical early metastasis. The aim was to describe the prevalence and distribution patterns of HPV DNA...

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Published in:Journal of medical virology 2008-04, Vol.80 (4), p.694-701
Main Authors: Landro, María Eulalia, Dalbert, Delia, Picconi, María Alejandra, Cúneo, Nicasio, González, Joaquín, Vornetti, Silvia, Bazán, Graciela, Mural, Juan, Basiletti, Jorge, Teyssié, Angélica Rita, Alonio, Lidia Virginia
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creator Landro, María Eulalia
Dalbert, Delia
Picconi, María Alejandra
Cúneo, Nicasio
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Mural, Juan
Basiletti, Jorge
Teyssié, Angélica Rita
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description The metastasis status of pelvic lymph nodes (PLNs) seems to be a predictive factor of survival. It was suggested that the presence of HPV DNA and other biological markers in PLN may indicate a sub clinical early metastasis. The aim was to describe the prevalence and distribution patterns of HPV DNA and H‐ras mutations in intra operatively obtained cervical tumors and PLN. Thirty‐seven cervical tumors and 61 lymph node biopsies from 37 patients with cervical cancer were selected. HPV typing and location were performed by PCR/dot blot and in situ hybridization (ISH) respectively. PCR/RFLP was used to scan for mutations in H‐ras. Hundred percent of the cervical cancers and 85% of the PLN were HPV positive; co‐infection with more than one type was 27%. HPV 16 was detected alone or co‐infecting with other types in 84% of tumors and 46% of PLN; the second most frequent viral type was HPV 18 (tumor: 27%; PLN: 20%). In PLN, HPV was located in nuclei or/and cytoplasm of lymphocytes, macrophages, endothelial, and /or stromal cells. H‐ras mutations were identified in 5/24 (21%) of patients with cervical tumors showing poor or moderated differentiation. HPV DNA in histological tumor‐free PLN not necessary indicate metastasis, but it may be associated to an active immune reaction. Mutated H‐ras is probably involved in cervical carcinogenesis and its detection in tumor and metastasis free PLN may be related to early metastasis or recurrence in at least a subset of poorly differentiated cervical tumors. J. Med. Virol. 80:694–701, 2008. © 2008 Wiley‐Liss, Inc.
doi_str_mv 10.1002/jmv.21076
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ispartof Journal of medical virology, 2008-04, Vol.80 (4), p.694-701
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1096-9071
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subjects Adult
Aged
Biological and medical sciences
Cell Nucleus - virology
cervical cancer
co-factor
Cytoplasm - virology
DNA, Viral - genetics
Endothelial Cells - virology
Female
Follow-Up Studies
Fundamental and applied biological sciences. Psychology
Genes, ras
H-ras oncogene
HPV
Human papillomavirus
Human papillomavirus 16 - isolation & purification
Human papillomavirus 18 - isolation & purification
Human viral diseases
Humans
In Situ Hybridization
Infectious diseases
lymph node
Lymph Nodes - virology
Lymphocytes - virology
Macrophages - virology
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Mutation
Papillomavirus Infections - virology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Retrospective Studies
Stromal Cells - virology
subclinical metastasis
Uterine Cervical Neoplasms - virology
Viral diseases
Virology
title Human papillomavirus and mutated H-ras oncogene in cervical carcinomas and pathological negative pelvic lymph nodes: A retrospective follow-up
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