Hyperbaric oxygen-induced attenuation of hemorrhagic transformation after experimental focal transient cerebral ischemia

• Published in: Stroke (1970) 2007-04, Vol.38 (4), p.1362-1367
• Main Authors: ZHIYONG QIN, KARABIYIKOGLU, Murat, YA HUA, SILBERGLEIT, Robert, YANGDONG HE, KEEP, Richard F, GUOHUA XI
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blood-Brain Barrier - drug effects; Blood-Brain Barrier - physiopathology; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Cerebral Hemorrhage - etiology; Cerebral Hemorrhage - physiopathology; Cerebral Hemorrhage - prevention & control; Cerebral Infarction - complications; Cerebral Infarction - physiopathology; Disease Models, Animal; Fibrinolytic Agents - adverse effects; Fundamental and applied biological sciences. Psychology; Hemoglobins - metabolism; Hyperbaric Oxygenation - methods; Infarction, Middle Cerebral Artery - complications; Infarction, Middle Cerebral Artery - physiopathology; Ischemic Attack, Transient - complications; Ischemic Attack, Transient - physiopathology; Male; Medical sciences; Neurology; Neuropharmacology; Neuroprotective agent; Oxygen - pharmacology; Oxygen - therapeutic use; Pharmacology. Drug treatments; Rats; Rats, Sprague-Dawley; Reperfusion Injury - complications; Reperfusion Injury - physiopathology; Tissue Plasminogen Activator - adverse effects; Treatment Outcome; Vascular diseases and vascular malformations of the nervous system; Vertebrates: nervous system and sense organs
• ISSN: 0039-2499; 1524-4628
• DOI: 10.1161/01.str.0000259660.62865.eb

An increased risk of hemorrhagic transformation is a major factor limiting the use of tissue plasminogen activator for stroke. Increased hemorrhagic transformation is also found in animals undergoing transient focal cerebral ischemia with hyperglycemia; this study examined whether hyperbaric oxygen (HBO) could reduce such hemorrhagic transformation in a rat model. Rats received an injection of 50% glucose (6 mL/kg intraperitoneally) and had a middle cerebral artery occlusion 10 minutes later. Rats were treated with HBO (3 ATA for 1 hour) 30 minutes after middle cerebral artery occlusion. Control rats received normobaric room air. Rats underwent reperfusion 2 hours after middle cerebral artery occlusion. Blood-brain barrier permeability (Evans blue), hemorrhagic transformation (hemoglobin content), brain edema, infarct volume, and mortality were measured. HBO treatment reduced Evans blue leakage in the ipsilateral hemisphere (28.4+/-3.5 versus 71.8+/-13.1 microg/g in control group, P