Evaluation of the chemical constituents and the antimicrobial activity of the volatile oil of Citrus reticulata fruit (Tangerine fruit peel) from South West Nigeria

The volatile oil of tangerine fruit (Citrus reticulata) was extracted by steam distillation and assessed for antibacterial and antioxidant activity. The volatile oil was tested against some Gram-negative organisms (Escherichia coli ATCC 35218, E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae,...

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Bibliographic Details
Published in:African journal of biotechnology 2008-07, Vol.7 (13), p.2227-2231
Main Authors: Ayoola, G A, Johnson, O O, Adelowotan, T, Aibinu, I E, Adenipekun, E, Adepoju-Bello, A A, Coker, HAB, Odugbemi, TO
Format: Article
Language:English
Subjects:
Candida albicans
Citrobacter
Citrus reticulata
Enterococcus faecalis
Escherichia coli
Klebsiella pneumoniae
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella paratyphi
Staphylococcus aureus
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Summary:The volatile oil of tangerine fruit (Citrus reticulata) was extracted by steam distillation and assessed for antibacterial and antioxidant activity. The volatile oil was tested against some Gram-negative organisms (Escherichia coli ATCC 35218, E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella paratyphi, Proteus mirabilis and Citrobacter spp); Gram-positive organisms such as Staphylococcus aureus ATCC 25923, S. aureus, Enterococcus faecalis and a fungus (Candida albicans). The minimum inhibitory concentration (MIC) was determined with concentrations of oil extract ranging from 0.87 to 445 mg/ml. Result of the study showed that the oil has a broad spectrum antibacterial activity. MIC recorded were S. aureus (0.74 mg/ml), S. aureus ATTC 25923 (2.46 mg/ml), E. faecalis (1.26 mg/ml), S. typhi (2.07 mg/ml), K. pneumoniae (0.56 mg/ml), E. coli ATTC 35218 (0.19 mg/ml), E. coli (1.95 mg/ml), P. aeruginosa (0.97 mg/ml), C. albicans (0.68 mg/ml). Antioxidant screening with 2,2-diphenyl-1-picrylhydrazyl (DPPH) was negative. Analysis of the chemical constituent by GC-MS showed the presence of D-limonene as the major constituent. Other constituents found were a-pinene and b-pinene.
ISSN:1684-5315
1684-5315