Application of a Novel Hydrophilic Infrared-Transparent Membrane to the Differentiation Between Microcolonies of Enterobacter Sakazakii and Klebsiella Pneumonia

A proof-of-concept study is reported for the differentiation between microcolonies of Enterobacter sakazakii and Klebsiella pneumoniae by means of a novel sample preparation for infrared (IR) analysis. A disposable, IR-transparent, microporous (0.2- mu m pores), hydrophobic, polyethylene (PE) membra...

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Bibliographic Details
Published in:Journal of food protection 2007-01, Vol.70 (5), p.1241-1245
Main Authors: Mossoba, M M, Al-Khaldi, S F, Curtis, S K, Battrell, C F, Fry, F S
Format: Article
Language:English
Subjects:
Enterobacter sakazakii
Klebsiella pneumoniae
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Summary:A proof-of-concept study is reported for the differentiation between microcolonies of Enterobacter sakazakii and Klebsiella pneumoniae by means of a novel sample preparation for infrared (IR) analysis. A disposable, IR-transparent, microporous (0.2- mu m pores), hydrophobic, polyethylene (PE) membrane (51 mu m thick) was plasma treated under an oxygen atmosphere and used to (i) filter (or print microarrays of) dilute aqueous foodborne bacterial suspensions and (ii) subsequently grow bacterial microcolonies when the treated, hydrophilic PE membrane was placed over brain heart infusion agar medium and incubated. Because this unique membrane is transparent to IR light, isolated microcolonies (200 mu m) of bacterial cells grown on this PE substrate for the first time could be directly fingerprinted by IR microspectroscopy in the transmission mode. Hence, time-consuming bacterial cell transfer from culture plates to an IR sample holder for subsequent measurement by IR spectroscopy was eliminated. Multivariate analysis of the observed IR spectra for microcolonies allowed the rapid differentiation between E. sakazakii and K. pneumoniae.
ISSN:0362-028X