Ameliorative effect of ajwain extract on hexachlorocyclohexane-induced lipid peroxidation in rat liver

• Published in: Food and chemical toxicology 2009-02, Vol.47 (2), p.279-282
• Main Authors: Anilakumar, K.R., Saritha, V., Khanum, Farhath, Bawa, A.S.
• Format: Article
• Language: English
• Subjects: Ajwain extract; Animal Feed; Animals; Antioxidants - pharmacology; Apiaceae - chemistry; Biological and medical sciences; Bone Marrow Cells - drug effects; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - prevention & control; Cytoprotection; Eating; Hexachlorocyclohexane; Insecticides - toxicity; Lindane - toxicity; Lipid peroxidation; Lipid Peroxidation - drug effects; Liver; Liver - drug effects; Liver - metabolism; Male; Medical sciences; Medicine, Ayurvedic; Micronuclei, Chromosome-Defective - chemically induced; Micronucleus Tests; Oxidative Stress - drug effects; Oxidoreductases - metabolism; Pesticides, fertilizers and other agrochemicals toxicology; Plant Extracts - pharmacology; Rat; Rats; Rats, Wistar; Toxicology
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2008.09.061

Effect of ajwain extract on hexachlorocyclohexane-induced oxidative stress and toxicity in rats were investigated. Six groups of rats were maintained for 12 weeks as (1) Control; (2) HCH (300 mg/kg body weight) injected (3) 1% ajwain extract incorporated diet (4)1% ajwain extract incorporated diet + HCH (5) 2% ajwain extract incorporated diet and (6) 2% ajwain extract incorporated diet + HCH. Results revealed that HCH administration lead to an increase in hepatic lipid peroxidation associated with reduction in, levels of glutathione (GSH), activity of superoxide dismutase (SOD), catalase and glucose-6-phosphate dehydrogenase. Prefeeding of ajwain extract resulted in decreased hepatic levels of lipid peroxides and increased GSH, GSH-peroxidase, G-6-PDH, SOD, catalase and glutathione S-transferase (GST) activities. At the same time there was a significant reduction in hepatic levels of HCH-induced raise in lipid peroxides as a result of the prefeeding the extract. Prefeeding of ajwain extract at 1% level to rats injected with HCH reverted the significant changes in catalase, G-6-PDH, GST and -glutamyl transpeptidase. HCH-induced formation of micronuclei in femur bone marrow was also reduced significantly. It was concluded that HCH administration resulted in hepatic free radical stress, causing toxicity, which could be reduced by the dietary ajwain extract.