Characterization of the Caenorhabditis elegansIslet LIM-homeodomain ortholog, lim-7

lim-7 is one of seven Caenorhabditis elegans LIM-homeodomain (LIM-HD)-encoding genes and the sole Islet ortholog. LIM-HD transcription factors, including Islets, function in neuronal and non-neuronal development across diverse phyla. Our results show that a lim-7 deletion allele causes early larval...

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Bibliographic Details
Published in:FEBS letters 2009-01, Vol.583 (2), p.456-464
Main Authors: Voutev, R, Keating, R, Jane Albert Hubbard, E, Vallier, L G
Format: Article
Language:eng ; jpn
Subjects:
Caenorhabditis
Caenorhabditis elegans
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Summary:lim-7 is one of seven Caenorhabditis elegans LIM-homeodomain (LIM-HD)-encoding genes and the sole Islet ortholog. LIM-HD transcription factors, including Islets, function in neuronal and non-neuronal development across diverse phyla. Our results show that a lim-7 deletion allele causes early larval lethality with terminal phenotypes including uncoordination, detached pharynx, constipation and morphological defects. A lim-7(+) transgene rescues lethality but not adult sterility. A lim-7(+) reporter in the full genomic context is expressed in all gonadal sheath cells, URA neurons, and additional cells in the pharyngeal region. Finally, we identify a 45-bp regulatory element in the first intron that is necessary and sufficient for lim-7 gonadal sheath expression.
ISSN:0014-5793
DOI:10.1016/j.febslet.2008.12.046