Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex

Dopamine receptor expression is correlated with certain types of cancers, including lung, breast and colon cancers. In this study, we report luminescent iridium(iii) complexes ( ) as intracellular dopamine receptor (D1R/D2R) cell imaging agents. Complexes and , which are conjugated with a dopamine r...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2018-02, Vol.9 (5), p.1119-1125
Main Authors: Vellaisamy, Kasipandi, Li, Guodong, Ko, Chung-Nga, Zhong, Hai-Jing, Fatima, Sarwat, Kwan, Hiu-Yee, Wong, Chun-Yuen, Kwong, Wai-Jing, Tan, Weihong, Leung, Chung-Hang, Ma, Dik-Lung
Format: Article
Language:English
Subjects:
Cells (biology)
Chemistry
Chromophores
Colon
Dopamine
Drug addiction
Fluorescence
Imaging
Iridium
Iridium compounds
Luminescence
Microscopy
Receptors
Toxicity
Tracking
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Summary:Dopamine receptor expression is correlated with certain types of cancers, including lung, breast and colon cancers. In this study, we report luminescent iridium(iii) complexes ( ) as intracellular dopamine receptor (D1R/D2R) cell imaging agents. Complexes and , which are conjugated with a dopamine receptor agonist, showed superior cell imaging characteristics, high stability and low cytotoxicity (>100 μM) in A549 lung cancer cells. siRNA knockdown and dopamine competitive assays indicated that complexes and could selectively bind to dopamine receptors (D1R/D2R) in A549 cells. Fluorescence lifetime microscopy demonstrated that complex has a longer luminescence lifetime at the wavelength of 560-650 nm than DAPI and other chromophores in biological fluids. The long luminescence lifetime of complex not only provides an opportunity for efficient dopamine receptor tracking in biological media, but also enables the temporal separation of the probe signal from the intense background signal by fluorescence lifetime microscopy for efficient analysis. Complex also shows high photostability, which could allow it to be employed for long-term cellular imaging. Furthermore, complex could selectively track the internalization process of dopamine receptors (D1R/D2R) in living cells. To the best of our knowledge, complex is the first metal-based compound that has been used to monitor intracellular dopamine receptors in living cells.
ISSN:2041-6520
2041-6539
DOI:10.1039/c7sc04798c