Possible association of oestrogen and Cryba4 with masticatory muscle tendon‐aponeurosis hyperplasia

Objective Masticatory muscle tendon‐aponeurosis hyperplasia, which is associated with limited mouth opening, progresses very slowly from adolescence. The prevalence rates of this disease are higher among women than among men, suggesting oestrogen involvement. As parafunctional habits are frequently...

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Bibliographic Details
Published in:Oral diseases 2019-01, Vol.25 (1), p.274-281
Main Authors: Hayashi, N, Sato, T, Kokabu, S, Usui, M, Yumoto, M, Ikami, E, Sakamoto, Y, Nifuji, A, Hayata, T, Noda, M, Yoda, T
Format: Article
Language:English
Subjects:
Adolescents
Animals
Aponeurosis - pathology
beta-Crystallin A Chain - genetics
Cell proliferation
Cells, Cultured
Cryba4
Crystallin
Dentistry
differentiation
Estrogens
Estrogens - physiology
Humans
Hyperplasia
Mastication
masticatory muscle tendon‐aponeurosis hyperplasia
Masticatory Muscles - pathology
Mechanical loading
Mice
oestrogen
Stress, Mechanical
Tendons - pathology
tenocyte
tensile force
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Summary:Objective Masticatory muscle tendon‐aponeurosis hyperplasia, which is associated with limited mouth opening, progresses very slowly from adolescence. The prevalence rates of this disease are higher among women than among men, suggesting oestrogen involvement. As parafunctional habits are frequently observed, mechanical stress is likely involved in the pathogenesis and advancement of this disease. To elucidate the pathological condition, we examined the effect of oestrogen on tenocyte function and the relationship between mechanical stress and crystallin beta A4 (Cryba4), using murine TT‐D6 tenocytes. Materials and Methods Cell proliferation assays, RT‐PCR, real‐time RT‐PCR, Western blot analysis and mechanical loading experiments were performed. Results The physiological dose of oestrogen increased the levels of scleraxis and tenomodulin in TT‐D6 tenocytes. In contrast, forced expression of Cryba4 inhibited scleraxis expression in these cells. Surprisingly, oestrogen significantly promoted cell differentiation in the Cryba4‐overexpressing TT‐D6 tenocytes. Moreover, tensile force induced Cryba4 expression in these tendon cells. Conclusion Oestrogen and Cryba4 may be associated with the progression of masticatory muscle tendon‐aponeurosis hyperplasia.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.12876