Cinnamic acid attenuates quorum sensing associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1

Objective Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against Pseudomonas aeruginosa was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein. Results At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-depend...

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Bibliographic Details
Published in:Biotechnology letters 2018-07, Vol.40 (7), p.1087-1100
Main Authors: Rajkumari, Jobina, Borkotoky, Subhomoi, Murali, Ayaluru, Suchiang, Kitlangki, Mohanty, Saswat Kumar, Busi, Siddhardha
Format: Article
Language:English
Subjects:
Acids
Animals
Anti-Bacterial Agents - pharmacology
Applied Microbiology
Bacterial Proteins - pharmacology
Biochemistry
Biofilms
Biofilms - drug effects
Biomedical and Life Sciences
Biotechnology
Caenorhabditis elegans - drug effects
Caenorhabditis elegans - microbiology
Cinnamates - pharmacology
Cinnamic acid
Detection
In vivo methods and tests
Inhibitors
Life Sciences
Ligands
Microbiology
Models, Molecular
Original Research Paper
Pathogens
Planktonic cells
Protective Agents - pharmacology
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Quorum Sensing - drug effects
RhlR protein
Transcription factors
Viability
Virulence
Virulence factors
Virulence Factors - metabolism
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Summary:Objective Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against Pseudomonas aeruginosa was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein. Results At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in P. aeruginosa without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development. In addition, the in vivo study indicated that the test compound protected Caenorhabditis elegans from the virulence factors of P. aeruginosa leading to reduced mortality. The in silico analysis revealed that Cinnamic acid can act as a competitive inhibitor for the natural ligands towards the ligand binding domain of the transcriptional activators of the quorum sensing circuit in P. aeruginosa , LasR and RhlR. Conclusions The findings suggest that Cinnamic acid may serve as a novel quorum sensing based anti-infective in controlling P. aeruginosa infections.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-018-2557-9