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Cinnamic acid attenuates quorum sensing associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1
Objective Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against Pseudomonas aeruginosa was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein. Results At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-depend...
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| Published in: | Biotechnology letters 2018-07, Vol.40 (7), p.1087-1100 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c409t-dda4943bed246d62fca2c2bfac088f0ab8bdbe634ec78d6460389f4edf5643223 |
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| cites | cdi_FETCH-LOGICAL-c409t-dda4943bed246d62fca2c2bfac088f0ab8bdbe634ec78d6460389f4edf5643223 |
| container_end_page | 1100 |
| container_issue | 7 |
| container_start_page | 1087 |
| container_title | Biotechnology letters |
| container_volume | 40 |
| creator | Rajkumari, Jobina Borkotoky, Subhomoi Murali, Ayaluru Suchiang, Kitlangki Mohanty, Saswat Kumar Busi, Siddhardha |
| description | Objective
Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against
Pseudomonas aeruginosa
was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein.
Results
At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in
P. aeruginosa
without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development. In addition, the in vivo study indicated that the test compound protected
Caenorhabditis elegans
from the virulence factors of
P. aeruginosa
leading to reduced mortality. The in silico analysis revealed that Cinnamic acid can act as a competitive inhibitor for the natural ligands towards the ligand binding domain of the transcriptional activators of the quorum sensing circuit in
P. aeruginosa
, LasR and RhlR.
Conclusions
The findings suggest that Cinnamic acid may serve as a novel quorum sensing based anti-infective in controlling
P. aeruginosa
infections. |
| doi_str_mv | 10.1007/s10529-018-2557-9 |
| format | article |
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Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against
Pseudomonas aeruginosa
was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein.
Results
At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in
P. aeruginosa
without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development. In addition, the in vivo study indicated that the test compound protected
Caenorhabditis elegans
from the virulence factors of
P. aeruginosa
leading to reduced mortality. The in silico analysis revealed that Cinnamic acid can act as a competitive inhibitor for the natural ligands towards the ligand binding domain of the transcriptional activators of the quorum sensing circuit in
P. aeruginosa
, LasR and RhlR.
Conclusions
The findings suggest that Cinnamic acid may serve as a novel quorum sensing based anti-infective in controlling
P. aeruginosa
infections.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-018-2557-9</identifier><identifier>PMID: 29680931</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acids ; Animals ; Anti-Bacterial Agents - pharmacology ; Applied Microbiology ; Bacterial Proteins - pharmacology ; Biochemistry ; Biofilms ; Biofilms - drug effects ; Biomedical and Life Sciences ; Biotechnology ; Caenorhabditis elegans - drug effects ; Caenorhabditis elegans - microbiology ; Cinnamates - pharmacology ; Cinnamic acid ; Detection ; In vivo methods and tests ; Inhibitors ; Life Sciences ; Ligands ; Microbiology ; Models, Molecular ; Original Research Paper ; Pathogens ; Planktonic cells ; Protective Agents - pharmacology ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Quorum Sensing - drug effects ; RhlR protein ; Transcription factors ; Viability ; Virulence ; Virulence factors ; Virulence Factors - metabolism</subject><ispartof>Biotechnology letters, 2018-07, Vol.40 (7), p.1087-1100</ispartof><rights>Springer Science+Business Media B.V., part of Springer Nature 2018</rights><rights>Biotechnology Letters is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-dda4943bed246d62fca2c2bfac088f0ab8bdbe634ec78d6460389f4edf5643223</citedby><cites>FETCH-LOGICAL-c409t-dda4943bed246d62fca2c2bfac088f0ab8bdbe634ec78d6460389f4edf5643223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29680931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajkumari, Jobina</creatorcontrib><creatorcontrib>Borkotoky, Subhomoi</creatorcontrib><creatorcontrib>Murali, Ayaluru</creatorcontrib><creatorcontrib>Suchiang, Kitlangki</creatorcontrib><creatorcontrib>Mohanty, Saswat Kumar</creatorcontrib><creatorcontrib>Busi, Siddhardha</creatorcontrib><title>Cinnamic acid attenuates quorum sensing associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Objective
Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against
Pseudomonas aeruginosa
was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein.
Results
At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in
P. aeruginosa
without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development. In addition, the in vivo study indicated that the test compound protected
Caenorhabditis elegans
from the virulence factors of
P. aeruginosa
leading to reduced mortality. The in silico analysis revealed that Cinnamic acid can act as a competitive inhibitor for the natural ligands towards the ligand binding domain of the transcriptional activators of the quorum sensing circuit in
P. aeruginosa
, LasR and RhlR.
Conclusions
The findings suggest that Cinnamic acid may serve as a novel quorum sensing based anti-infective in controlling
P. aeruginosa
infections.</description><subject>Acids</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Applied Microbiology</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Biochemistry</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Caenorhabditis elegans - drug effects</subject><subject>Caenorhabditis elegans - microbiology</subject><subject>Cinnamates - pharmacology</subject><subject>Cinnamic acid</subject><subject>Detection</subject><subject>In vivo methods and tests</subject><subject>Inhibitors</subject><subject>Life Sciences</subject><subject>Ligands</subject><subject>Microbiology</subject><subject>Models, Molecular</subject><subject>Original Research Paper</subject><subject>Pathogens</subject><subject>Planktonic cells</subject><subject>Protective Agents - pharmacology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Quorum Sensing - drug effects</subject><subject>RhlR protein</subject><subject>Transcription factors</subject><subject>Viability</subject><subject>Virulence</subject><subject>Virulence factors</subject><subject>Virulence Factors - metabolism</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUFrFTEQx4Mo9rX6AbxIwIuX1SSbzW6O5VGtUGgPeg7ZZPJI2U3azEbotzePVxUED8Mc5jf_CfkR8o6zT5yx8TNyNgjdMT51YhjGTr8gOz6MfafGUb0kO8Yl7wapxRk5R7xnjOmRja_JmdBqYrrnO_K0jynZNTpqXfTUbhukajdA-lhzqStFSBjTgVrE7GKbePozlrpAckCDdVsuSG3ydI45xGWlIZfVbjEnGhO9Q6g-rznZBkGph5gyWnp3ecvfkFfBLghvn_sF-fHl6vv-uru5_fptf3nTOcn01nlvpZb9DF5I5ZUIzgon5naZTVNgdp5mP4PqJbhx8koq1k86SPBhULIXor8gH0-5DyU_VsDNrBEdLItNkCsawdpvyLFVQz_8g97nWlJ73ZGaeqmEGBrFT5QrGbFAMA8lrrY8Gc7M0Ys5eTHNizl6MbrtvH9OrvMK_s_GbxENECcA2ygdoPw9_f_UX2jjmpo</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Rajkumari, Jobina</creator><creator>Borkotoky, Subhomoi</creator><creator>Murali, Ayaluru</creator><creator>Suchiang, Kitlangki</creator><creator>Mohanty, Saswat Kumar</creator><creator>Busi, Siddhardha</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20180701</creationdate><title>Cinnamic acid attenuates quorum sensing associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1</title><author>Rajkumari, Jobina ; Borkotoky, Subhomoi ; Murali, Ayaluru ; Suchiang, Kitlangki ; Mohanty, Saswat Kumar ; Busi, Siddhardha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-dda4943bed246d62fca2c2bfac088f0ab8bdbe634ec78d6460389f4edf5643223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acids</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - 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Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajkumari, Jobina</au><au>Borkotoky, Subhomoi</au><au>Murali, Ayaluru</au><au>Suchiang, Kitlangki</au><au>Mohanty, Saswat Kumar</au><au>Busi, Siddhardha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cinnamic acid attenuates quorum sensing associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>40</volume><issue>7</issue><spage>1087</spage><epage>1100</epage><pages>1087-1100</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Objective
Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against
Pseudomonas aeruginosa
was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein.
Results
At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in
P. aeruginosa
without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development. In addition, the in vivo study indicated that the test compound protected
Caenorhabditis elegans
from the virulence factors of
P. aeruginosa
leading to reduced mortality. The in silico analysis revealed that Cinnamic acid can act as a competitive inhibitor for the natural ligands towards the ligand binding domain of the transcriptional activators of the quorum sensing circuit in
P. aeruginosa
, LasR and RhlR.
Conclusions
The findings suggest that Cinnamic acid may serve as a novel quorum sensing based anti-infective in controlling
P. aeruginosa
infections.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29680931</pmid><doi>10.1007/s10529-018-2557-9</doi><tpages>14</tpages></addata></record> |
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| subjects | Acids Animals Anti-Bacterial Agents - pharmacology Applied Microbiology Bacterial Proteins - pharmacology Biochemistry Biofilms Biofilms - drug effects Biomedical and Life Sciences Biotechnology Caenorhabditis elegans - drug effects Caenorhabditis elegans - microbiology Cinnamates - pharmacology Cinnamic acid Detection In vivo methods and tests Inhibitors Life Sciences Ligands Microbiology Models, Molecular Original Research Paper Pathogens Planktonic cells Protective Agents - pharmacology Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Quorum Sensing - drug effects RhlR protein Transcription factors Viability Virulence Virulence factors Virulence Factors - metabolism |
| title | Cinnamic acid attenuates quorum sensing associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1 |
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