Shoot formation from root tip region: a developmental alteration by WUS in transgenic tobacco

We examined the effect of ectopic expression of WUS on the morphology of tobacco seedlings and the segments in vitro. WUS was amplified from Arabidopsis cDNA and introduced into the tobacco genome under the transcriptional control of the β-estradiol-inducible expression system. When 1-week-old trans...

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Bibliographic Details
Published in:Plant cell reports 2007-09, Vol.26 (9), p.1449-1455
Main Authors: Rashid, Syeda Zinia, Yamaji, Naoki, Kyo, Masaharu
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis Proteins - metabolism
Biological and medical sciences
Biotechnology
Ectopic expression
Estradiol - pharmacology
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Plant - drug effects
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Nicotiana - drug effects
Nicotiana - genetics
Nicotiana tabacum
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - drug effects
Plant Roots - physiology
Plant Shoots - drug effects
Plant Shoots - growth & development
Plants, Genetically Modified
Root tip
Seedlings
Seedlings - drug effects
Seedlings - metabolism
Shoot formation
Tobacco
Transdifferentiation
Transgenes
WUSCHEL
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Summary:We examined the effect of ectopic expression of WUS on the morphology of tobacco seedlings and the segments in vitro. WUS was amplified from Arabidopsis cDNA and introduced into the tobacco genome under the transcriptional control of the β-estradiol-inducible expression system. When 1-week-old transgenic seedlings were cultured in the presence of β-estradiol, only the root tip region developed bulbous tissues followed by shoot formation and plant regeneration, suggesting its applicability for improving the strategy of micropropagation in recalcitrant species. Evident abnormality was not observed in the cotyledons, hypocotyl nor root except for the tip. However, ectopic WUS seemed to be functional in those parts through the observation of gene expression and the behavior of cultured segments. Small root segments with a root tip treated with β-estradiol also showed bulbing but no shoots unless exogenous cytokinin was supplied. These findings suggest the existence of unknown factors regulating ectopic WUS function in the seedling.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-007-0342-7