Rapid, cost-effective liquid chromatograghic method for the determination of metronidazole in biological fluids

A rapid and cost effective method for the analysis of metronidazole in biological samples was developed. The extraction method is a simple single-step liquid-liquid process that has eliminated the need for costly extraction and evaporation equipment. The mobile phase consists largely of water, makin...

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Bibliographic Details
Published in:African journal of biotechnology 2006-07, Vol.5 (13), p.1188-1190
Main Authors: Mustapha, K B, Odunola, M T, Garba, M, Obodozie, O
Format: Article
Language:English
Online Access:Get full text
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Summary:A rapid and cost effective method for the analysis of metronidazole in biological samples was developed. The extraction method is a simple single-step liquid-liquid process that has eliminated the need for costly extraction and evaporation equipment. The mobile phase consists largely of water, making the method cheap to run with less than 6 min total analytical time per sample. The calibration curve was linear from 0 to 2.00 mu g/ml. The regression coefficient was 0.99. The method is highly sensitive, with limit of detection of 1 ng/ml. The coefficient of variation for within-day run was less than 4% while that of day-to-day run was less than 6%. There were no interfering peaks from endogenous materials in the serum. The method was validated and used for pharmacokinetic studies.
ISSN:1684-5315
1684-5315