Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography

• Published in: Biotechnology progress 2018-07, Vol.34 (4), p.999-1005
• Main Authors: Saavedra, Soledad L., Martínez Ceron, María C., Giudicessi, Silvana L., Forno, Guillermina, Bosco, María Belén, Marani, Mariela M., Erra‐Balsells, Rosa, Albericio, Fernando, Cascone, Osvaldo, Camperi, Silvia A.
• Format: Article
• Language: English
• Subjects: Affinity chromatography; Amino acids; Animals; biologics; Chemical synthesis; Chromatography, Affinity - methods; Cow's milk; downstream processing; Growth hormones; Human Growth Hormone - chemistry; Human Growth Hormone - isolation & purification; Humans; Milk; Milk - chemistry; Organic chemistry; peptide ligands; Peptides; Protein Array Analysis; Purification; recombinant biopharmaceuticals; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; solid‐phase peptide synthesis; transgenic cow milk
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1002/btpr.2645

Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:999–1005, 2018