Notch activation promotes osteoblast mineralization by inhibition of apoptosis

Notch activator Jagged1 (JAG1) plays a critical role in the regulation of osteoblast differentiation and bone metabolism. In this study, JAG1‐induced osteoblast proliferation, differentiation, and mineralization has been analyzed in primary osteoblasts for up to 7 days. Alkaline phosphatase and Aliz...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular physiology 2018-10, Vol.233 (10), p.6921-6928
Main Authors: Xu, Ying, Shu, Bing, Tian, Ye, Chelly, Marjorie, Morandi, Massimo M., Barton, Shane, Shang, Xifu, Dong, Yufeng
Format: Article
Language:English
Subjects:
Activation
Alizarin
Alkaline phosphatase
Apoptosis
Biocompatibility
Bone turnover
Cbfa-1 protein
Cell culture
Cell proliferation
Clonal deletion
Differentiation
Flow cytometry
Jagged1 protein
Metabolism
Mineralization
mRNA
Notch protein
notch signaling
osteoblast differentiation
Osteoblastogenesis
Osteoblasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Notch activator Jagged1 (JAG1) plays a critical role in the regulation of osteoblast differentiation and bone metabolism. In this study, JAG1‐induced osteoblast proliferation, differentiation, and mineralization has been analyzed in primary osteoblasts for up to 7 days. Alkaline phosphatase and Alizarin red staining showed an enhanced osteoblast maturation and mineralization in JAG1 treated cells, as well as higher mRNA levels of late osteoblast differentiation markers. In contrast, Notch inhibitor DAPT and deletion of Runx2 totally blocked JAG1 effects on osteoblast mineralization. Flow cytometry data further showed a significantly higher cell proliferation in early stages of culture at day 3, and lower levels of osteoblast apoptosis in late stages of culture at day 7. More importantly, activation of anti‐apoptotic factor BCL‐2 was enhanced, while pro‐apoptotic factor Caspase3 was reduced in JAG1 treated osteoblasts. Therefore, we conclude that cell mineralization is enhanced via anti‐apoptotic actions of Notch signaling within the osteoblast cells. JAG1‐mediated Notch activation induced osteoblast mineralization. This enhanced cell mineralization was modulated through decreased cell apoptosis in a caspase3‐dependent manner.
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.26592