Pseudopropionibacterium rubrum sp. nov., a novel red‐pigmented species isolated from human gingival sulcus

• Published in: Microbiology and immunology 2018-06, Vol.62 (6), p.388-394
• Main Authors: Saito, Masanori, Shinozaki‐Kuwahara, Noriko, Tsudukibashi, Osamu, Hashizume‐Takizawa, Tomomi, Kobayashi, Ryoki, Kurita‐Ochiai, Tomoko
• Format: Article
• Language: English
• Subjects: Acetic acid; Acetic Acid - metabolism; Acids; Bacterial Proteins - genetics; Bacterial Typing Techniques; Base Composition; Benzoquinones - analysis; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - genetics; Fatty Acids - analysis; Fermentation; Genes; Genes, Bacterial; Gingiva - microbiology; Glucose - metabolism; gram‐positive rod; Homology; Humans; Lactic acid; Lactic Acid - metabolism; New species; Nucleic Acid Hybridization; Nucleotide sequence; oral microbiota; Phylogeny; Propionates - metabolism; Propionibacteriaceae - classification; Propionibacteriaceae - genetics; Propionibacteriaceae - isolation & purification; Propionibacteriaceae - metabolism; Propionic acid; Pseudopropionibacterium rubrum; RNA polymerase; RNA, Ribosomal, 16S - genetics; RpoB protein; rRNA 16S; Sequence Analysis, DNA; Sequence Homology; Species Specificity; Strain; Succinic acid; Succinic Acid - metabolism
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/1348-0421.12592

ABSTRACT In this study, sStrain SK‐1T, a novel gram‐positive, pleomorphic, rod‐shaped, non‐spore forming, non‐motile organism, designated SK‐1T, was isolated from human gingival sulcus and found to produce acetic acid, propionic acid, lactic acid, and succinic acid as end products of glucose fermentation. Strain SK‐1T is most closely related to Pseudopropionibacterium (Propionibacterium) propionicum with sequence homologies of the 16S rRNA and RNA polymerase β subunit (rpoB) genes of 96.6% and 93.1%, respectively. The genomic DNA G + C content of the isolate was 61.8 mol%. On the basis of the sequence data of the 16S rRNA and housekeeping (rpoB) genes, a novel taxon is here proposed, Pseudopropionibacterium rubrum sp. nov. (type strain SK‐1T = JCM 31317T = DSM 100122T). The 16S rRNA and rpoB gene sequences of strain SK‐1T have been deposited in the DDBJ under the accession numbers LC002971 and LC102236, respectively.