The immune functions of sessile hemocytes in three organs of kuruma shrimp Marsupenaeus japonicus differ from those of circulating hemocytes

Shrimp, as invertebrates, have an open vasculature that allows circulating hemocytes to infiltrate the tissues, where they are referred to as sessile hemocytes. Sessile hemocytes are known to express immune-related genes, but it is not known whether their functions differ from those of circulating h...

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Bibliographic Details
Published in:Fish & shellfish immunology 2018-07, Vol.78, p.109-113
Main Authors: Koiwai, Keiichiro, Kondo, Hidehiro, Hirono, Ikuo
Format: Article
Language:English
Subjects:
Immune related genes
Marsupenaeus japonicus
Sessile hemocytes
Shrimp
Transcriptome analysis
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Summary:Shrimp, as invertebrates, have an open vasculature that allows circulating hemocytes to infiltrate the tissues, where they are referred to as sessile hemocytes. Sessile hemocytes are known to express immune-related genes, but it is not known whether their functions differ from those of circulating hemocytes. To answer this question, we enriched them from suspensions of different tissues using discontinuous density gradient centrifugation and analyzed their transcripts by RNA-seq. The results suggest that circulating hemocytes and sessile hemocytes of the gills are in a state that could react quickly to pathogens, immune-related genes expression of sessile hemocytes differ from circulating hemocytes, and the gills, heart and lymphoid organs have cells that express immune-related genes that are different from hemocytes. •Sessile hemocytes of gills, heart and lymphoid organs were enriched.•Transcriptome analysis between circulating and sessile hemocytes was conducted.•Sessile hemocytes may have a different type of hemocytes from circulating hemocytes.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2018.04.036