Neonatal whisker trimming in WAG/Rij rat pups causes developmental delay, encourages maternal care and affects exploratory activity in adulthood

• Published in: Brain research bulletin 2018-06, Vol.140, p.120-131
• Main Authors: Smirnov, Kirill, Tsvetaeva, Daria, Sitnikova, Evgenia
• Format: Article
• Language: English
• Subjects: Animals; Anxiety; Avoidance Learning; Behavior, Animal; Body Weight; Brain - physiology; Compulsive Behavior; Conditioning (Psychology); Development; Developmental Disabilities - etiology; Disease Models, Animal; Epilepsy, Absence; Exploratory activity; Exploratory Behavior; Fear; Female; Male; Maternal behavior; Maternal Behavior - physiology; Motor Skills; Rats; Sensory deprivation; Sensory experience; Vibrissae
• ISSN: 0361-9230; 1873-2747
• DOI: 10.1016/j.brainresbull.2018.04.010

•Maternal behavior in rat females is sensitive to whisker trimming of their pups.•Neonatal whisker trimming caused deficiency in body weight in adolescents, but not in adults.•Development of early behavioral functions delayed after neonatal whisker trimming.•Neonatal whisker trimming affected exploratory activity in adults.•Neonatal whisker trimming did not affect fear-based learning and anxiety in adults. WAG/Rij rats are genetically predisposed to absence epilepsy. Maternal behavior in WAG/Rij female rats is known to differ from that in non-epileptic females. We hypothesize that (1) mother’s behavior may be changed as response to changes in pup’s conditions; (2) sensory deprivation at the neonatal age affect learning and behavior in adulthood. All whiskers in WAG/Rij rat pups were trimmed daily during PN1-PN8. Maternal behavior was examined during the same period. It was found that in the control group, WAG/Rij females often demonstrated abnormally long (>1 min) repetitive purposeless stereotypical actions that were roughly classified as compulsive-like behavior. Mothers of the trimmed pups showed less compulsive-like behavior and more intensively interacted with pups and built better nests. Rat pups in the trimmed group had lower body weight on PN7-PN19 as compared to the control. In the trimmed group, maturation of motor skills and early behavioral patterns (i.e. walking, grooming, vertical activity, motor functions of forelimbs) showed 1–2 days delay in comparison to the control. At the age of 2–2.5 months, the locomotor activity in the trimmed rats differed from the control, but the level of anxiety was the same (the open field and the elevated plus maze). At the age of 6 months, the trimmed and control rats showed no differences in conditioned avoidance learning test, therefore, neonatal whisker trimming did not influence fear-based learning abilities in adulthood. It is hypothesized that an enhanced maternal care is capable to modulate development of brain functions in sensory deprived progeny.